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Integrated extraction technique for sea cucumber polypeptide and polysaccharide

A sea cucumber polypeptide and comprehensive extraction technology, which is applied in fermentation and other directions, can solve the problems of single sea cucumber active substances and sea cucumber protein toxic substances, and achieve the effects of retaining active ingredients, overcoming toxic substances, and improving yield and purity

Inactive Publication Date: 2008-06-04
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the deficiencies of the above-mentioned prior art, and provide a comprehensive extraction process of sea cucumber polypeptides and polysaccharides, which mainly solves the problem that the existing extraction process can only obtain a single sea cucumber active substance, and the polypeptide contains large molecular weight substances. Peptide and chemical hydrolysis of sea cucumber protein is prone to produce toxic substances

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1, using fresh sea cucumber produced in Zhifu Island, Yantai, Shandong Province as raw material, washed to remove sediment and internal organs, put into -70 ℃ ultra-low temperature refrigerator for quick freezing for 4.5 hours, cut into pieces and put into a meat grinder to grind. Then put it into a colloid mill to grind it into a colloid, heat the sea cucumber in a colloidal state at 55°C for 25 minutes, then add a fully automatic hydrolysis reactor, add deionized water and compound trypsin, and the amount of deionized water is 100% for cleaning. 6 times the weight of the fresh sea cucumber in the viscera, hydrolyzed, after the hydrolysis, high-temperature sterilization to kill enzymes, take out the hydrolyzed solution and centrifuge to remove sediment and insoluble matter, add 95% ethanol to the system ethanol volume fraction is 60%, centrifuge after standing still for 12 hours, put on The supernatant liquid is a mixture of water-soluble sea cucumber polypeptid...

Embodiment 2

[0040] Example 2, using fresh sea cucumber produced in Zhifu Island, Yantai, Shandong Province as raw material, washed to remove sediment and internal organs, put into -70 ℃ ultra-low temperature refrigerator for quick freezing for 4 hours, cut into pieces and put into a meat grinder to grind. Then put it into a colloid mill to grind it into a colloid, heat the sea cucumber in a colloidal state at 50°C for 30 minutes, then add a fully automatic hydrolysis reactor, add deionized water and compound trypsin, the amount of deionized water is 100% for cleaning and removing 5 times the weight of the fresh sea cucumber in the viscera, hydrolyzed, high-temperature sterilization after hydrolysis, remove the hydrolyzed solution and centrifuge to remove sediment and insoluble matter, add 95% ethanol to the ethanol volume fraction of the system to 60%, centrifuge after standing still for 12 hours, put on The supernatant liquid is a mixture of water-soluble sea cucumber polypeptides; the ce...

Embodiment 3

[0043] Example 3, using fresh sea cucumber produced in Zhifu Island, Yantai, Shandong Province as raw material, washed to remove sediment and viscera, put into -70 ℃ ultra-low temperature refrigerator for quick freezing for 5 hours, cut into pieces and put into a meat grinder to grind. Then put it into a colloid mill to grind it into a colloid, heat the sea cucumber in a colloidal state at 70°C for 20 minutes, then add a fully automatic hydrolysis reactor, add deionized water and compound trypsin, and the amount of deionized water is 100% for cleaning. 7 times the weight of the fresh sea cucumber in the viscera, hydrolyze it, sterilize it at high temperature after the hydrolysis, take out the hydrolyzate and centrifuge to remove the precipitate and insoluble matter, add 95% ethanol until the volume fraction of ethanol in the system is 60%, centrifuge after standing still for 12 hours, and put it on The supernatant liquid is a mixture of water-soluble sea cucumber polypeptides; ...

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Abstract

The invention discloses a comprehensive extraction process of holothurian polypeptides and polysaccharides. The invention is characterized in that: fresh holothurians are taken as raw materials, gelatinized and hydrolyzed under the effect of composite trypsinases; precipitation and supernatant are respectively collected through centrifugation of the holothurians; ethanol is added into the supernatant for centrifugation; supernatant obtained is mixture of the holothurian polypeptides; centrifugal precipitations obtained for two times are synthesized, and A.S1398 purified neutral proteases are added for hydrolysis and centrifugation; ethanol is added into the supernatant for centrifugation; supernatant obtained for two times is synthesized into holothurian polypeptide mixture, and precipitation is crude polysaccharides; the holothurian polypeptide mixture is dissolved in water, and ultrafiltration membranes with different apertures are used for separation and purification of subsections of different molecular weights; holothurian polypeptide powders of different molecular weight sections are obtained after debitterizing, decolorization and freeze drying; crude polysaccharides are mixed into 5 percent aqueous solution; after centrifugation, decolorization and repeated centrifugation, precipitation is colleted and washed, and aqueous solution is mixed and dialyzed; ethanol is added for repeated precipitation for at least three times, and purified holothurian polysaccharides are obtained. The invention simultaneously prepares the holothurian polypeptides with different molecular weight sections and the holothurian polysaccharides, and has the advantages of high yield, high purity, high safety and mild reaction conditions.

Description

technical field [0001] The invention relates to the technical field of development and application of marine organisms, in particular to a comprehensive extraction process of sea cucumber polypeptides and polysaccharides. Background technique [0002] In recent years, with the application of modern science and technology at home and abroad on the identification and classification of sea cucumber varieties, the physiology and biochemistry of sea cucumbers, the separation and identification of biologically active substances in sea cucumbers, and their biomedical effects, it has been found that sea cucumbers contain many important Biologically active substances, such as polypeptides, sea cucumber polysaccharides, sea cucumber saponins, sea cucumber toxins, etc. Modern nutritional studies have shown that low molecular weight peptides can be directly absorbed by the intestinal mucosa without being digested by the stomach. In addition, sea cucumber peptides also have the effects ...

Claims

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Application Information

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IPC IPC(8): C12P21/00C12P19/04
Inventor 付学军金海珠王洪涛
Owner OCEAN UNIV OF CHINA
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