Device for detecting hepatitis b virus marker in mouth cavity liquid

A technology of hepatitis B virus and detection device, which can be applied to measurement devices, instruments, scientific instruments, etc., can solve problems such as unreported and applied problems, and achieve the effect of high sensitivity

Inactive Publication Date: 2008-05-21
马北峰
View PDF0 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no similar reports and applications

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Device for detecting hepatitis b virus marker in mouth cavity liquid

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0033] Colloidal gold-labeled mouse monoclonal anti-s and anti-e were prepared by the Roth J method. The glass fiber membrane pad was pre-treated with bovine serum albumin (BSA), and then the gold-labeled anti-s and anti-e mixed gold-labeled pad was prepared and placed in the antigen. There are 10 gold standard pads. Another mouse monoclonal anti-s, anti-e dot membrane was placed on antigen detection area 16 and antigen detection area 17, and antigen control line 14 and antibody control line 15 were goat anti-mouse IgG antibody and anti-human IgG antibody. Antigen indicating line 12, antibody indicating line 13, indicator is CaSO4. Gold-labeled Staphylococcus protein A (40nm) was placed on the antibody gold-labeled pad at 11 places. Spot HbsAg, HBeAg and HbcAg on the solid-phase membrane to form antibody detection 1 area 18 , antibody detection 2 area 19 , and antibody detection 3 area 20 . Oral fluid collection pad 2 is a cellulose filter paper salt pad, that is, soak the m...

example 2

[0036] 1) Prepare anti-s, anti-e and anti-c-biotin conjugates respectively, and combine them with biotin-N-hydroxySuccinimide ester.

[0037] 2) Prepare hepatitis B antigen detection: gold label pad (anti-s, anti-e mixed) is prepared as in previous example 1, use streptavidin 6mg / ml in Tris butter to spot the membrane in the middle of Tris butter for antigen detection 16. Antigen detection Zone 2, 17, after drying, biotinylated monoclonal anti-s and anti-e (1.0 mg / ml) were directly spotted on streptavidin respectively. The remaining steps are the same as Example 1, and the control is goat anti-mouse IgG antibody.

[0038] 3) Preparation of hepatitis B antibody detection (competition method): first prepare HBsAg, HBeAg and HBcAg mixed gold label pad, similar to the above, first use streptavidin to form antibody detection area 18, antibody detection area 2 area 19, and antibody detection area 3 Dot the membrane on 20, and then use step 1 to prepare biotinylated anti-s, anti-e a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention provides a detecting device for the hepatitis B virus marker in oral cavity liquid, which relates to a device which can quickly detect the hepatitis B virus marker in the oral cavity liquid. The present invention consists of an external lid which is a oral cavity liquid gathering part, a shell upper layers of test paper which are overlapped together, a piece of test paper and the lower layer of the test paper. The present invention comprises the oral cavity liquid sample gathering part which can complete by directly gathering the oral cavity liquid through putting the paper into mouth or by using a gatherer, and reaction and result observation part which comprises antigen, antibody reaction and observation part. If the gathered oral cavity liquid has corresponding antigen, a composite is formed by the antigen, golden dot antibody, and over-membrane, a red line appears positive (sandwich method). The corresponding antibody can be detected in the same way. Or the antigen and the antibody can be detected by a competition method. And the detecting sensitivity of the sample is obviously increased by combining biotin and affinity element amplification system. The present invention can detect the hepatitis b virus marker in the oral cavity liquid quickly and conveniently and is provided with high sensitivity and specificity.

Description

technical field [0001] The invention relates to a device capable of rapidly detecting hepatitis B virus markers in oral fluid, and simultaneously detecting multiple indicators of hepatitis B antigens and antibodies. Background technique [0002] At present, the detection of markers of hepatitis B virus (hereinafter referred to as hepatitis B) in oral fluid—antigens and antibodies—is mostly used by experimental researchers, and there is no report on its application in clinical medicine. The methods used were enzyme-linked immunosorbent assay (Elisa), modified Elisa and radioimmunoassay (RIA). These methods require complex instruments and equipment, cumbersome operation steps, professional operation, high cost, long time, and usually need to wait several hours to several days for the result, and the radioactive substances in the RIA method will pollute the environment. [0003] Unlike blood samples, oral fluids are low-volume secretions with high concentrations of mucin and o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/576G01N33/558
Inventor 马北峰马北阳
Owner 马北峰
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap