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Preparation method of immobilization bacterium for restoring polycyclic aromatic hydrocarbons contaminated soil

A technology of polluted soil and polycyclic aromatic hydrocarbons, applied in the restoration of polluted soil, immobilized on or in the inorganic carrier, etc., can solve problems such as failure to achieve, improve immobilization efficiency, improve diffusion and mass transfer performance, The effect of a good living environment

Inactive Publication Date: 2008-05-14
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although immobilization technology has been widely used in the remediation of polluted fluid media (such as wastewater) and semi-fluid media (such as mud), it has not been successfully applied in the remediation of polluted non-fluid media (such as soil).
Therefore, it is widely used to remediate polluted fluid media (such as wastewater) and semi-fluid media (such as mud) environments, but the immobilized microorganisms are used to degrade organic pollutants in non-fluid media (such as soil). Not yet reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Liquid culture of immobilized bacteria

[0025] In seed medium (glucose 1.25%, beef extract 0.25%, NH 4 NO 3 0.1%, MgSO 4 7H 2 O 0.02%, KCl 0.02%, pH 6.5) Erlenmeyer flasks were inoculated with Bacillus, and placed in a constant temperature shaker at 28-30°C for 12 hours.

[0026] (2) Pretreatment of immobilized carrier

[0027] The red brick powder sieved through a 1.00mm sieve is soaked in tap water at a ratio of 0.5 to 1.5ml / g for 12 to 24 hours, sterilized by high-temperature steam at 121°C for 90 minutes, and set aside.

[0028] (3) Preparation of immobilized particles

[0029] Get the bacterial suspension, add evenly to the surface of the aseptic carrier, inoculum size 2%, put it into a 28°C constant temperature incubator for dark cultivation after sealing, add the proliferation medium (glucose 4.0% , yeast paste 0.3%, KH 2 PO 4 0.05%, MgSO 4 ·7H 2 O 0.025%, (NH 4 ) 2 HPO 4 0.2%, pH 7.0-7.2), after 3 days, immobilized bacterial particles were ...

Embodiment 2

[0034] The difference from Example 1 is that during the pretreatment of the carrier, the fly ash sieved through a 0.25mm sieve was soaked in tap water at a ratio of 1.5ml / g for 12h, sterilized by high-temperature steam at 121°C for 90min, and set aside.

[0035]The prepared immobilized particles were put into the soil polluted by polycyclic aromatic hydrocarbons according to the dosage ratio of 6% of the soil weight. After 42 days, the removal rate of PYR in the soil was 42%; the removal rate of BaP in the soil was 36%. During the whole degradation process, the degradation rate of the immobilized particles was fast, and the immobilized particles still had more than 90% activity after 42 days.

Embodiment 3

[0037] The difference from Example 1 is that the vermiculite passed through a 1mm sieve was soaked with tap water at a ratio of 0.5ml / g for 24 hours, sterilized by high-temperature steam at 121°C for 90 minutes, and set aside.

[0038] The prepared immobilized particles were put into the soil polluted by polycyclic aromatic hydrocarbons according to the ratio of 6% of the soil weight. After 42 days, the removal rate of PYR in the soil was 51%; the removal rate of BaP in the soil was 39%. During the whole degradation process, the degradation rate of the immobilized particles was fast, and the immobilized particles still had more than 90% activity after 42 days.

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Abstract

The invention relates to cell immobilization technology, in particular to a preparation method for immobilized bacteria used in the restoration of polycyclic aromatic hydrocarbons contaminated soil, using 0.25-1.00mm red brick powder, fly ash, vermiculite, peat soil, diatoms Soil or coke powder is used as the carrier material. After sterilization, it is inserted into the bacterial solution, and then multiplied and cultivated to obtain the desired immobilized particles. The immobilized biological product prepared according to the formula of the invention has a good remediation effect on soil polluted by high-molecular-weight polycyclic aromatic hydrocarbons, and is a reliable new environment-friendly biological product.

Description

technical field [0001] The invention relates to cell immobilization technology, in particular to a method for preparing immobilized granules for repairing polycyclic aromatic hydrocarbons (PAHs) polluted soil, which uses red brick powder, coke powder, fly ash, vermiculite, peat Soil, diatomaceous earth and other materials as the carrier of adsorption immobilization microorganism technology. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous in the environment and are often adsorbed on soil particles due to their high octanol-water partition coefficient and poor water solubility. Therefore, soil becomes the main carrier of PAHs. The accumulation of polycyclic aromatic hydrocarbons in nature will cause great harm to human beings and the whole nature. After some polycyclic aromatic hydrocarbons enter the human body and animals, they will cause carcinogenic, teratogenic and mutagenic consequences. Therefore, the remediation technology of PAHs-...

Claims

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Application Information

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IPC IPC(8): C12N11/14B09C1/10
Inventor 苏丹李培军鞠京丽
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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