Apple polysaccharide, its preparation method and application in preventing and treating tumor metastasis medicine

A tumor metastasis, apple technology, applied in anti-tumor drugs, drug combinations, pharmaceutical formulations and other directions, can solve the problems of difficult to widely use, difficult for patients to tolerate, unsatisfactory synergy, etc., and achieves a simple and easy to operate preparation method. Side effects, effects of rich apple resources

Inactive Publication Date: 2008-04-30
刘莉 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Tumor metastasis is the main cause of death caused by tumors, but anti-tumor metastasis drugs are very scarce, especially among the existing drugs, chemotherapy drugs that inhibit tumor metastasis have serious side effects and are difficult for patients to tolerate; various chemotherapy drugs cooperate with each other and have synergistic effects Not ideal; patients take one or more drugs for a long time, on the one hand, it is difficult to tolerate, on the other hand, the tumor is prone to develop drug resistance to chemotherapy drugs
[0004] At present, only modified pectin (MCP) derived from American tangerine can target galectin-3, but due to its limited source and high production cost, it is difficult to be widely used

Method used

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  • Apple polysaccharide, its preparation method and application in preventing and treating tumor metastasis medicine

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Experimental program
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Effect test

Embodiment 1

[0019] Step (1): Prepare apple crude polysaccharide: take the apple pomace that has been squeezed, crush it, and reflux it with ethanol for 3 times, each time for 8 hours. The residue is refluxed with water for 3 times, each time for 4 hours. Repeated freezing and thawing to remove protein, activated carbon decolorization, dialysis, concentration, freeze-drying, to obtain.

[0020] Step (2): Mix 15g of apple crude polysaccharide with methanol solution of 2mol / L NaOH, stir vigorously, heat and reflux at 60-80°C for 4-6h, cool to room temperature, add concentrated hydrochloric acid to adjust the pH value to 3, stir vigorously, 50- Heat and reflux at 70°C for 7-10 hours, evaporate the solvent under reduced pressure, add an appropriate amount of distilled water to the concentrated suspension until it is fully dissolved, transfer it to a dialysis bag (molecular weight cut-off 8000), use distilled water as the external fluid for dialysis, until the color of the dialysis internal flui...

Embodiment 2

[0023] Step (1): Prepare apple crude polysaccharide: take the apple pomace that has been squeezed, crush it, and reflux the ethanol twice for 10 hours each time, and reflux the residue through water for 3 times for 3.5 hours each time, collect the reflux liquid, concentrate, and precipitate with ethanol, Repeated freezing and thawing to remove protein, activated carbon decolorization, dialysis, concentration, freeze-drying, to obtain.

[0024] Step (2): Mix 15g of apple crude polysaccharide with methanol solution of 2mol / L NaOH, stir vigorously, heat and reflux at 60-80°C for 4-6h, cool to room temperature, add concentrated hydrochloric acid to adjust the pH value to 3, stir vigorously, 50- Heat and reflux at 70°C for 7-10 hours, evaporate the solvent under reduced pressure, add an appropriate amount of distilled water to the concentrated suspension until it is fully dissolved, transfer it to a dialysis bag (molecular weight cut-off 8000), use distilled water as the external fl...

Embodiment 3

[0027] Step (1): Prepare apple crude polysaccharide: take the apple pomace that has been squeezed, crush it, and reflux the ethanol for 3 times, 7 hours each time, and reflux the residue 3 times with water, 4.5 hours each time, collect the reflux liquid, concentrate, ethanol precipitation, Repeated freezing and thawing to remove protein, activated carbon decolorization, dialysis, concentration, freeze-drying, to obtain.

[0028] Step (2): Add 2g of apple crude polysaccharide in batches to 400mL of 0.5M HCl / imidazole solution (pH=7), stir vigorously until the phases of the solution are uniform, and weigh 10g of NaBH 4 Add a small amount to the above solution several times, stir vigorously at room temperature for 4 hours, add 25 mL of acetic acid to terminate the reaction, continue stirring for 30 minutes, add sufficient amount of absolute ethanol to form a colloidal precipitate, filter, wash the precipitate twice with 60% ethanol and 5% HCl, The precipitate was washed twice wit...

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Abstract

The invention relates to the technical field of drugs for preventing and inhibiting tumor metastasis, in particular to an apple polysaccharide, its preparation method and its application in drugs for preventing and inhibiting tumor metastasis. One of the objects of the present invention is to provide a modified apple polysaccharide (MAP) used as a drug for preventing and inhibiting tumor metastasis. The second object is to provide a preparation method for the above-mentioned apple polysaccharide. And the application of anti-tumor metastasis drugs to fill the serious shortage of anti-tumor metastasis drugs. Technical solution provided by the present invention: an apple polysaccharide prepared by the following method, comprising the following steps: (1) obtaining apple crude polysaccharide; (2) reducing the degree of esterification so that the degree of esterification is less than 20% Then through gel chromatography or molecular weight cutoff, the apple polysaccharide with a molecular weight of 5-15 kDa is obtained.

Description

Technical field: [0001] The invention relates to the technical field of drugs for preventing and inhibiting tumor metastasis, in particular to an apple polysaccharide, its preparation method and its application in drugs for inhibiting tumor metastasis. Background technique: [0002] Galectin-3 is a member of the galectin family, widely present in normal cells, highly expressed in a variety of tumor cells, and significantly increased during cancer metastasis and tumor invasion. The biggest feature of Galectin-3 is that it can bind to ligands containing galactose residues. Many oncogenes in the body such as MUC1 and carcinoembryonic antigens such as Tn antigen have galactose residues, which can combine with galectin-3 to promote tumor metastasis. In colon cancer, infected cells lose their ability to regulate growth, forming tumors at certain sites. When cancer cells enter the bloodstream, mucin forms a protective film that prevents cancer cells from spreading and becoming tu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61K31/715A61P35/04C08B37/00C12P19/04A61K131/00
Inventor 刘莉梅其炳白卉李宇华孙阳
Owner 刘莉
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