Method for ferment production of 2.3-butanediol by directly enzymolysis of plants stalk

Abstract

The invention relates to a method for producing 2,3-dihydroxybutane with fermentation through direct enzymatic hydrolysis of the stalk of plant, which comprises the following steps that: a bacillus with high cellulose activity and capable of producing 2,3-dihydroxybutane is separated from the polluted soil around the blanching workshop of Rong Cheng, Shang Dong Province, with the stalk or the wooden fiber as the sole carbon source, the bacillus is used to carry out solid fermentation to produce 2,3-dihydroxybutane. Aiming to exploit the substitute of the oil to solve the problem of the shortage of oil with the increasing price, the invention utilizes the renewable resource such as stalk to produce the key platform chemical of 2,3-dihydroxybutane through microorganism fermentation to accomplish the high-value utilization of the substances of the stalk, and overcome the puzzle of the method to produce the 2,3-dihydroxybutane at present, such as the impossibility of direct usage of cellulose as the carbon source, the fermentation inhibitor which is easily produced during the chemical-physical pretreatment, and the hard extraction due to the liquid fermentation.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for producing 2.3-butanediol by direct fermentation of straw without adding cellulase by using a bacillus strain capable of secreting cellulase. Background technique [0002] 2.3-Butanediol, as a potentially very valuable platform compound, has attracted widespread attention internationally. It is a colorless and odorless chiral compound with a molecular weight of 90.12kDa, a high boiling point (180-184°C), and a low freezing point (-60°C). It is an excellent antifreeze agent. As a chemical intermediate, it can be dehydrated into diacetyl, which is widely used in fuel additives with high combustion value, and 1.3-butadiene, which is widely used in synthetic rubber; other compounds can be generated through condensation, polymerization and other reactions , such as: styrene, octane and 2.3-butanediol diacetate, etc., as additives can be widely used in ink...

AI Technical Summary

Problems solved by technology

[0006] Although it is economically feasible to use lignocellulosic waste as a carbon source from natural renewable resources, due to the high price of cellulase and the problems of fermentation inhibitors in hydrolyzate caused by physical and chemical pretreatment, cellulosic wastes can be It is difficult to industrialize the production of 2.3-butanediol by microbial fermentation from renewable resources

In view of this, the present invention discloses a method for producing 2.3-butanediol through microbial liquid and solid-state fermentation directly using straw as the sole carbon source without adding cellulase, which can solve the problem of producing 2.3-butanediol , it is necessary to add cellulase or use physical and chemical pretreatment, which often leads to the problem of fermentation inhibitors in the sugar liquid; through solid-state fermentation, it can also solve the problem of low concentration of 2.3-butanediol during liquid fermentation, difficult distillation extraction and high extraction cost.

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