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Composite protecting agent and uses thereof

A technology of protective agent and high molecular polymer, which is used in biological testing, material inspection products, etc., can solve the problems of not fully meeting the needs of production, reporting of protective agents, and unspecified synergistic effects of protective agents. Effect

Inactive Publication Date: 2008-02-06
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the existing chloramphenicol rapid test gold standard test strips generally have a shelf life of 3 to 12 months at 0-4 °C, which cannot fully meet the needs of production.
[0007] There have been some patents on the development of chloramphenicol gold standard test strips, but none of them involve the content of product protection agents; secondly, in the similar products of small molecule compound rapid test gold standard test strips, the issue of protective agents has not been reported yet; Furthermore, some foreign scholars have proposed a gold immune test strip for the diagnosis of macromolecular substances with a single protective agent, but they have not specified the synergistic effect of different combinations of protective agents.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1, a kind of composite protecting agent, make according to the following steps:

[0023] (1), the preparation of PBS buffer solution:

[0024] K H 2 PO 4 : 0.27g, anhydrous NaHPO 4 : 1.14g, NaCl: 8.00g and KCl: 0.20g, dissolved in distilled water and adjusted to 1L.

[0025] (2), preparation of compound protective agent:

[0026] Sucrose: 10.00g, trehalose: 5.00g, PEG (20000): 0.05g, BSA: 1.00g, Tween-20: 0.05g, sodium azide: 0.01g, dissolved in PBS buffer solution and adjusted to 100mL.

[0027] The above-mentioned composite protective agent is used to protect the chloramphenicol quick test gold standard test strip, and the specific preparation method is as follows:

[0028] (1) Treatment of chloramphenicol gold-labeled antibody

[0029] Take 5 mL of the treated colloidal gold solution, add 250 μg of chloramphenicol monoclonal antibody, mix well, centrifuge for several times, wash and purify, and separate the gold-labeled antibody precipitate. Take 1m...

Embodiment 2

[0044] Embodiment 2, a kind of composite protecting agent, make according to the following steps:

[0045] (1), the preparation of PBS buffer solution:

[0046] K H 2 PO 4 : 0.27g, anhydrous NaHPO 4 : 1.14g, NaCl: 8.00g, KCl: 0.20g, dissolved in distilled water and adjusted to 1L.

[0047] (2), preparation of compound protective agent:

[0048] Sucrose: 5.00g, trehalose: 2.00g, BSA: 0.50g, PVP (40000): 0.25g, Tween-20: 0.05g, sodium azide: 0.01g, dissolved in PBS buffer solution and adjusted to 100mL.

[0049] The above-mentioned composite protective agent is used to protect the chloramphenicol quick test gold standard test paper strip, and the specific preparation method is the same as in Example 1.

[0050] The prepared chloramphenicol gold standard test strips were subjected to an accelerated storage test, and the test method was the same as in Example 1. See Table 3 and Table 4 for specific results.

[0051] Table 3 Chloramphenicol Gold Standard Test Strip Accelerat...

Embodiment 3

[0058] Embodiment 3, a kind of composite protecting agent, make according to the following steps:

[0059] (1), the preparation of PBS buffer solution:

[0060] K H 2 PO 4 : 0.27g, anhydrous NaHPO 4 : 1.14g, NaCl: 8.00g, KCl: 0.20g, dissolved in distilled water and adjusted to 1L.

[0061] (2), preparation of compound protective agent:

[0062] Sucrose: 10.00g, BSA: 0.25g, PVP(40000): 0.10g, PEG(20000): 0.05g, Tween-20: 0.2g, sodium azide: 0.005g, dissolved in PBS buffer solution and adjusted to 100mL .

[0063] The above-mentioned composite protective agent is used to protect the chloramphenicol quick test gold standard test paper strip, and the specific preparation method is the same as in Example 1.

[0064] The prepared chloramphenicol gold standard test strips were subjected to an accelerated storage test, and the test method was the same as in Example 1. The specific results are shown in Table 5 and Table 6.

[0065] Table 5 Chloramphenicol Gold Standard Test Str...

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PUM

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Abstract

The present invention discloses a compound protective solute which is formed by the dissolution of glucide, high molecular polymer, protein and antiseptic in the buffer solution. The densities of glucide, high molecular polymer, protein and antiseptic are 1-20g / 100mL, 0.05-2.0g / 100mL, 0.1-2g / mL and 0.005-0.1g / mL respectively. The compound protective solute of the present invention is used to protect the chloromycetin rapid testing gold-labeled test strip; and can prolong the retention period of the test strip under zero to four DEG C by at least two years.

Description

technical field [0001] The invention belongs to the scope of auxiliary agents for the preservation of colloidal gold immunoassay test paper products, and in particular relates to a composite protective agent for gold standard test paper strips for rapid detection of chloramphenicol. Background technique [0002] Gold immunochromatography technology is a rapid solid-phase membrane immunoassay technology combined with colloidal gold labeling technology and protein chromatography technology, which is based on microporous membrane. It has been widely used in biomedicine, animal and plant quarantine, food safety supervision, etc. Many fields. Since the 1990s, the research on colloidal gold immunochromatographic rapid diagnostic test strips (referred to as gold standard test strips) has developed rapidly. Used to detect small molecule substances such as drugs of abuse, narcotics, stimulants, etc. In addition, gold standard test strips are increasingly playing a significant role ...

Claims

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Application Information

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IPC IPC(8): G01N33/52G01N33/53
Inventor 郭逸蓉王美英王姝婷杨挺朱国念
Owner ZHEJIANG UNIV
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