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Process for producing porous object and porous object obtained by the same

A manufacturing method and technology of porous bodies, applied in the field of porous bodies, can solve the problems of small pore size, difficulty in achieving a wide range of pore size, and difficulty in ensuring uniform pore distribution of porous bodies

Inactive Publication Date: 2010-09-01
JMS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method has the following problems: the production process becomes complicated because the elution of the particles is necessary, and it is difficult to ensure the uniformity of the pore distribution of the obtained porous body due to the sedimentation of the particles in the polymer solution.
However, even with this method, the obtained pore size is only about 50 to 80 μm, and it is difficult to realize a wide range of pore sizes.
In addition, as a freezing method, the rapid freezing method using liquid nitrogen is generally used, and there is a tendency for the pore size to become smaller

Method used

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  • Process for producing porous object and porous object obtained by the same
  • Process for producing porous object and porous object obtained by the same
  • Process for producing porous object and porous object obtained by the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The water content in the mixed solution was changed to produce a porous body, and the control of the pore size was confirmed.

[0054] A lactide-caprolactone copolymer (P(LA / CL=50 / 50)) with a composition ratio (molar ratio) of L-lactide and ε-caprolactone of 50:50, 1,4- Dioxane and water were mixed to prepare 29 kinds of mixed solutions with different water contents. In addition, the mixing ratio (mass ratio) of (P(LA / CL=50 / 50)) and 1,4-dioxane is a certain value (4:96), and the mixing ratio of water in the aforementioned mixed solution (containing water rate) changed to 1, 2, 4, 6, 8, 8.25, 8.5, 8.75, 9, 9.25, 9.5, 9.75, 10, 10.25, 10.5, 10.75, 11, 11.25, 11.5, 11.75, 12, 12.25, 12.5, 12.75, 13, 14, 16, 18, 20% by mass. Then, these mixed solutions (20 g) were supplied to stainless steel petri dishes (diameter 5 cm, depth 1.5 cm, the same below).

[0055] Place the aforementioned stainless steel Petri dish on a cooling rack (at room temperature) in a freeze dryer (tr...

Embodiment 2

[0065] The cooling rate was changed to make a porous body, and the control of the pore size was confirmed.

[0066] In addition to using P (LA / CL=51 / 49) in which the composition ratio of L-lactide and ε-caprolactone is 51:49, the same operation as in the aforementioned Example 1 was used to prepare a variety of different water contents. The solutions were mixed, and the aforementioned mixed solutions (20 g) were supplied to stainless steel petri dishes, respectively. Then, the aforementioned stainless steel culture dish was placed on a cooling rack of a freeze dryer (trade name TF 5-85ATANCS: manufactured by Takara Seisakusho), and the temperature of the cooling rack was lowered to 10°C (required time 25 minutes) and left for 60 minutes. After standing, the cooling rack was cooled to -50°C at a predetermined rate (180°C / hr, 10°C / hr, 5°C / hr, 3°C / hr), and treated at -50°C for 180 minutes. Then, at the same time as the cooling treatment was completed, the temperature in the free...

Embodiment 3

[0069] Investigate the effect of changes in freezing temperature on pore size.

[0070] The cooling rate is set to 180°C / hr, and the final cooling temperature is set to a predetermined temperature (-20°C, -30°C, -40°C), except that, the same operation as the above-mentioned Example 2 is performed to prepare a porous body sample, and Pore ​​size was determined (n=3). The relationship between the water content in the aforementioned mixed solution and the pore size of the aforementioned sample is shown in image 3 .

[0071] as it should image 3 It can be seen that when the cooling rate is 180°C / hr, the behavior of the pore size corresponding to the water content does not change greatly due to the change of the final cooling temperature, and is not affected by the aforementioned cooling temperature. From this, it can be said that as long as the cooling temperature is set at -20°C, excessive cooling is unnecessary and cost reduction can be achieved.

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Abstract

A process for porous-object production which enables the regulation of pore sizes, especially the regulation of not only small pore sizes but large pore sizes. The process comprises preparing a mixture solution comprising one or more polymers comprising a copolymer of lactide and caprolactone, a solvent in which the polymers have relatively low solubility, and a solvent in which the polymers haverelatively high solubility and which is compatible with the solvent in which the polymers have low solubility and freeze-drying the mixture solution to produce a porous object, wherein the content ofthe solvent in which the polymers have relatively low solubility in the mixture solution is changed and the freezing is conducted by cooling the mixture solution at a rate of 300 DEG C / hr or lower, whereby the pore size of the porous object is controlled. Thus, a porous object having pore sizes of 30-1,800 mu m is obtained.

Description

technical field [0001] The present invention relates to a method for producing a porous body, particularly a porous body used as a scaffold material for cells in the medical field centering on tissue engineering and regenerative medicine engineering. Background technique [0002] In the fields of tissue engineering and regenerative medical engineering, scaffolds are generally used to proliferate cells, and particularly in recent years, porous bodies made of bioabsorbable materials are expected to be used as scaffolds. In the case of such a bioabsorbable porous body, cells are seeded in the pores to proliferate, and then transplanted into a living body to cause tissue regeneration in the living body, and the bioabsorbable material used as a scaffold It is slowly decomposed and absorbed in the living body. Therefore, scaffolds for cell proliferation can be directly transplanted into organisms together with proliferating cells. [0003] As a method for producing such a porous...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/08C08J9/28
CPCC12N5/0068C08J2201/048C08J2367/04A61L27/56C08J9/28C12N11/08C12N2533/40C12N11/096
Inventor 栈敷俊信井手纯一花木尚幸松浦洋治
Owner JMS CO LTD
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