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Compound of sphaeria sinensis and panax notoginseng extract and the application thereof in the preventing and treating of pulmonary fibrosis

A technology of Cordyceps sinensis and pulmonary fibrosis, which is applied in the direction of drug combination, medical preparations containing active ingredients, drug delivery, etc., and can solve the problems of unapplied Cordyceps sinensis or its fungi

Inactive Publication Date: 2007-10-31
苏州中药研究所有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In 2005, Tian Qiong found that the prescription of Gentiana purple flower and total saponins of Panax notoginseng could inhibit the fibrosis of chronic obstructive pulmonary disease (CN1698725), but the prescription did not use Cordyceps sinensis or its fungus
This patent uses the fermented product of Mortierella sinensis as the fermented product of Cordyceps sinensis for the first time, but the fermented product is only its mycelium and culture fluid, not the extract
In addition, there is no Panax notoginseng extract in the compound

Method used

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  • Compound of sphaeria sinensis and panax notoginseng extract and the application thereof in the preventing and treating of pulmonary fibrosis
  • Compound of sphaeria sinensis and panax notoginseng extract and the application thereof in the preventing and treating of pulmonary fibrosis
  • Compound of sphaeria sinensis and panax notoginseng extract and the application thereof in the preventing and treating of pulmonary fibrosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Embodiment 1. The preparation of Cordyceps sinensis extract

[0062] 1. Extracted from the fermented mycelia of Mortierella chinensis

[0063] (1). Take 10Kg of fermented mycelia of Mortierella sinensis, add 100Kg of deionized water, and extract at 95°C (±3) for 2 hours. Filtrate while hot with a 50μ filter membrane to obtain water extract 1; the residue is extracted twice with 60Kg deionized water at 95°C (±3), each time for 1 hour, and then filtered to obtain water extract 2 and 3;

[0064] (2). Combine the water extracts 1, 2 and 3, concentrate under reduced pressure until the specific density reaches 1.04, and obtain the water extract concentrate of Cordyceps sinensis;

[0065] (3).Add 50Kg of 95% ethanol to the residue after the above water extraction, reflux extraction at 80°C (±1°C) for 3 hours, and filter it with a 50μ filter while it is hot to obtain alcohol extract 1, and add 95% to the residue 25Kg of ethanol, reflux extraction at 80°C (±1°C) for 2 times, e...

example 2

[0072] Example 2. Preparation of Panax notoginseng extract

[0073] 1. Extract from Panax notoginseng root

[0074] (1). Get Radix Notoginseng crude drug (root) 10Kg, slice, pulverize, cross 8 mesh sieves;

[0075] (2) Add 10 times the amount of 70% ethanol, reflux extraction for 2 hours, and obtain alcohol extract 1 after filtration;

[0076] (3) The residue was reflux extracted with 8 times the amount of 70% ethanol twice, each time for 1 hour, and the ethanol extracts 2 and 3 were obtained after filtration;

[0077] (4). Alcoholic extracts 1, 2 and 3 were combined, concentrated under reduced pressure to obtain liquid extract;

[0078] (5). Add ion-free water to the liquid extract to dissolve, so that the concentration of the solution reaches 0.4 grams of crude drug per milliliter;

[0079] (6). The above-mentioned solution is loaded on the macroporous adsorption resin (HPD-100 type) chromatographic column, so that the saponin is adsorbed on the resin, the resin consumpti...

example 3

[0095] Example 3. Anti-pulmonary fibrosis assay

[0096] Mix the Cordyceps sinensis extract and Panax notoginseng extract prepared above in a ratio of 7:3, then use a dose of 0.6g / Kg as a therapeutic dose, and inject it into the lung fibrosis sensitized with bleomycin mice. On the 7th, 14th and 28th day after administration, the alveolitis and pulmonary fibrosis degree of pathological sections of each group and the hydroxyproline content in lung tissue were compared and observed, and compared with normal mice, bleomycin model group and Hormone therapy group compared.

[0097]Results: In the lung pathological sections of the model group on the 7th day, a large number of macrophages, neutrophils and plasma cells had exuded into the alveolar space, and alveolitis was obvious; Widening, massive proliferation of fibroblasts and collagen matrix; in the lung pathological section on day 28, part of the alveolar structure was destroyed, the alveolar cavity shrank, and the alveolar se...

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Abstract

The invention discloses a cordycepin extract and notoginseng extract and application in prevention and cure pulmonary fibrosis medicine preparation, which is characterized by the following: extracting and condensing from China clothing hairs pore zymocyte powder with water and alcohol; getting cordycepin extract; setting Cordyceps sinensis adenosine, cordycepic acid, and Cordyceps sinensis polysaccharide more than raw bacteria powder about 3-8 double; extracting cordycepin extract from cordycepin crude drug with alcohol; separating large aperture absorption resin; purifying; getting the cordycepin extract; setting the content of cordycepin saponin more than raw cordycepin crude drug for 7-10 double; allocating the cordycepin and cordycepin saponin with mass percent at 90-10% and 10-90%; preparing medicinal compound. This invention possesses very big utility value in clinic.

Description

Technical field: [0001] The invention relates to the technical field of traditional Chinese medicine. It specifically relates to the composition of Cordyceps sinensis and Panax notoginseng extract and its application in the preparation of medicines for preventing and treating pulmonary fibrosis. Background technique: [0002] Cordyceps sinensis refers specifically to an ascomycete that parasitizes on the larvae of the genus Hepialus. The so-called 'grass' is its sub-seat; 'worm' is its sclerotia, but the sclerotia is covered with a layer of insect corpse skin. [0003] Cordyceps sinensis mainly grows in alpine meadows on the Qinghai-Tibet Plateau of China at an altitude of 3500-5000 meters. It is a national second-class protected species. Due to over-excavation in modern times, resources have been nearly exhausted, and the price has become more expensive than gold. Isolation and artificial cultivation of Cordyceps sinensis has become an important task for today's scienti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/258A61K9/16A61K9/20A61K9/48A61K9/08A61P11/00A61K36/068
Inventor 杨庆尧顾振纶杨晓彤张永胜李强展端糜可冯慧琴
Owner 苏州中药研究所有限公司
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