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Application of end-grain cutting protein peptide A to stomach cancercell lymph node metastasis detection

A technology for fibrin and gastric cancer cells, which is applied in the field of protein fingerprinting and protein fingerprinting of biological sample analysis, which can solve the problems of limited gel capacity and sensitivity, complexity, and inability to observe molecular weight.

Inactive Publication Date: 2007-08-22
许洋
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

First, the method provides only two characteristics of biomolecules - mass and isoelectric point (pI)
Second, the resolution in each dimension is limited by the resolving power of the gel
For example, it is often difficult to distinguish between biomolecules that differ by less than 5% in mass or that differ in pI
Third, gels have limited capacity and sensitivity and may not be able to detect small amounts of expressed biomolecules
Fourth, small proteins or peptides with a molecular weight below about 10-20 kDa cannot be observed
Since human cell proteins are much more complex than potatoes, electrophoresis alone to identify protein fingerprints of diseases is far from meeting clinical needs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Serum in embodiment 1 gastric cancer telo-cut fibrinopeptide A Applications of Differential Expression

[0064] (1) Experimental method

[0065] 1. Materials

[0066] 1. Specimen sources: A. Serum from 46 normal controls; B. Serum from 20 gastric cancer patients without lymph node invasion; C. Serum from 58 gastric cancer patients with lymph node invasion.

[0067] 2. Quality control: A. Human standardized quality control serum B. Mass spectrometer laser energy regulation: Before each test, use the above-mentioned standardized quality control serum.

[0068] 2. Method

[0069] 1. Collection of samples: After the whole blood is collected, draw the serum and store it at -80°C; take out the serum sample in the refrigerator at -80°C, and put it on the ice box to melt; centrifuge at 10,000 rpm and 4°C for 2 minutes; take the supernatant liquid.

[0070]2. Sample preparation A: 3 μl of serum is required for each cationic adsorbent support point, and the serum is diluted ...

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Abstract

The invention relates to an albumen dactyloscopy analyzed by protein group. Utilizing mass spectrography to distinguish, the method discovers a new end-cut fiber albumen peptide A which can be used to test lymph node transfer of gastric cancer cells. The method is accurate, convenient and speed.

Description

technical field [0001] The invention relates to a novel method for analyzing proteins in biological samples, in particular to a protein fingerprint method for analyzing biological samples. The present invention also relates to protein fingerprinting in which the proteome is captured by retention chromatography and analyzed using a computer readable barcode format (protein fingerprint). Background technique [0002] Both normal function and pathological characteristics of cells depend to some extent on the function of proteins expressed by cells. Therefore, identifying differences in proteins expressed within cells can be used in the diagnosis of disease and ultimately in drug development and disease treatment. However, differential analysis of protein expression and function requires the ability to resolve complex mixtures of intracellular molecules. However, many substances in cells often exist in trace amounts. The current methods for analyzing proteins have limitations ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/60G01N33/68
Inventor 许洋
Owner 许洋
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