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Polyamide dendroid polymer nano particle loading survivin small molecule RNA

A polyamide branch and nanoparticle technology is applied in the field of new pharmaceutical dosage forms to achieve the effects of increasing intracellular concentration, increasing transfection rate, and good resistance to enzymes

Inactive Publication Date: 2010-05-19
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recently, polyamide-amine dendrimers (PAMAM dendrimers) used abroad have larger carrying capacity, stronger membrane penetration ability and more sustained-release performance than the previously used polylactic acid-glycolic acid copolymer (PLGA) and other nanocarriers. Good, harmless to human body and many other advantages, but so far there are no reports of polyamide-amine dendrimers carrying survivin-specific small molecule RNA at home and abroad.

Method used

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  • Polyamide dendroid polymer nano particle loading survivin small molecule RNA
  • Polyamide dendroid polymer nano particle loading survivin small molecule RNA
  • Polyamide dendroid polymer nano particle loading survivin small molecule RNA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Precisely extract 10D small RNA molecules by centrifugation and dilute with 50 microliters of ultrapure water to obtain solution A; take 16 microliters of the 7th generation polyamide-amine dendrimer solution and dilute with 34 microliters of ultrapure water to obtain solution B; Dilute 1 μl of B solution to 15 μl to obtain solution C; take 1 μl of A solution and 0.4 μl of C solution, add 18.6 μl of serum-free medium, and incubate at room temperature for 10 minutes to obtain survivin Small RNA polyamide dendrimer nanoparticles.

[0031] See the small RNA package situation image 3 .

Embodiment 2

[0033] Precisely extract 10D small RNA molecules by centrifugation and dilute with 50 microliters of ultrapure water to obtain solution A; take 16 microliters of the 7th generation polyamide-amine dendrimer solution and dilute with 34 microliters of ultrapure water to obtain solution B; Dilute 1 μl of B solution to 15 μl to obtain solution C; take 1 μl of A solution and 2 μl of C solution, add 17 μl of serum-free medium, and incubate at room temperature for 10 minutes to obtain survivin Small RNA polyamide dendrimer nanoparticles.

[0034] See the small RNA package situation image 3 .

Embodiment 3

[0036] Precisely extract 10D small RNA molecules by centrifugation and dilute with 50 microliters of ultrapure water to obtain solution A; take 16 microliters of the 7th generation polyamide-amine dendrimer solution and dilute with 34 microliters of ultrapure water to obtain solution B; Dilute 1 microliter of B solution to 15 microliters to obtain solution C; take 1 microliter of A solution and 4 microliters of C solution, add 15 microliters of serum-free medium, and incubate at room temperature for 10 minutes to obtain survivin Small RNA polyamide dendrimer nanoparticles.

[0037] See the small RNA package situation image 3 .

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Abstract

The invention relates to a method for preparing the carrier RNA polyamide dendric polymer nanometer particles. The invention is characterized in that it uses polyamide dendric polymer as carrier whoseamido group number is at 1:10 ratio with the phosphate group number of RNA, to form the polyamide dendric polymer RNA crosslink composite, while the average diameter is 85.64nm at 25Deg. C, with highpackage on RNA, high carriage, and improved anti-acid and anti-enzyme abilities of RNA, improved cell transfer rate, and the high-efficiency character of small molecule disturbance technique. The invention can be used in the treatment of liver cancer.

Description

Technical field [0001] The invention relates to a novel pharmaceutical dosage form, in particular to a survivin-carrying small molecule RNA polyamide dendrimer nanoparticle and a preparation method thereof. Background technique [0002] Survivin is an important apoptosis inhibitor newly discovered in recent years. It is selectively expressed in a variety of malignant tumors (especially liver cancer), but not in normal tissue cells. It is possible to inhibit the expression of survivin Promoting the apoptosis of liver cancer cells has become a new target for current malignant tumor treatment research. [0003] Antisense technology is an effective gene therapy method in tumor treatment research in recent years, and has important value for blocking harmful gene expression and blocking tumor occurrence and development. Among various antisense methods, the newly emerged ribonucleic acid interference (RNA interference, RNAi) technology is particularly eye-catching, which means that doubl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/34A61K9/14A61K48/00A61P35/00A61P1/16
Inventor 蔡洪培董勤
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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