Rhodotorula mucilaginosa for producing beta-caroten, beta-caroten and its production method

A technology of carotene and Torula rhodozyme, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problem of low β-carotene content, achieve short growth cycle, high yield, temperature and Effects over a wide pH range

Inactive Publication Date: 2007-08-01
XINJIANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently screened Rhodotorula strains have low β-carotene content, and the product has not yet been promoted

Method used

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  • Rhodotorula mucilaginosa for producing beta-caroten, beta-caroten and its production method
  • Rhodotorula mucilaginosa for producing beta-caroten, beta-caroten and its production method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Isolation and identification of Rhodotorula mucilaginosa CGMCC No.1536 producing β-carotene

[0029] 1. Sample collection

[0030] Soil samples were collected from abandoned gardens in Urumqi, Xinjiang, and separated on the surface of suitable yeast medium, such as YPD medium (glucose 20%, peptone 20%, yeast extract 10%), at 28°C after screening and optimization. The Rhodotorula mucilaginosa XJU-1 strain was obtained.

[0031] 2. Identification of strains

[0032] It was identified by molecular taxonomy based on the base sequence of the D1 / D2 region in the large subunit 26S rDNA and the base sequence analysis of the small subunit 5.8S rDNA and two internal transcribed spacers (ITS1 and ITS2). Combined with morphological observation and determination of physiological and biochemical characteristics, it was accurately identified as Rhodotorula mucilaginosa CGMCC No.1536.

Embodiment 2

[0033] Example 2: Cultivation of Rhodotorula mucilaginosa CGMCC No.1536 strain producing β-carotene and confirmation of fermentation process.

[0034] The selected medium is as follows:

[0035] ①Slant activation medium: glucose 2%; peptone 2%; yeast powder 1%; pH 9.0.

[0036] ② Liquid seed medium: 2% glucose; 2% peptone; 1% yeast powder; pH 8.5.

[0037] ③ Liquid fermentation medium: glucose 4%; peptone 2%; yeast powder 1%; pH 8.0.

[0038] The present invention adopts the conventional culture medium, but has special requirements on the pH. The conventional fermentation is carried out in an acidic environment, but the bacterial strain of the present invention produces high-yield beta-carotene in an alkaline condition.

[0039] Fermentation culture method (see accompanying drawing 1):

[0040] ① Slant Activation Culture: Transfer the strain from the preserved slant to the activation slant medium, and culture at 28°C for 48 hours.

[0041] ②Liquid seed culture: Received in...

Embodiment 3

[0044] Example 3: Fermentative production of β-carotene by Rhodotorula mucilaginosa CGMCC No.1536 strain.

[0045] Repeat the fermentation process steps in Example 2, and repeatedly verify the specific parameters in the fermentation process. The strain of the present invention preserved for a long time is inoculated on potato agar medium, at pH 7.0, temperature 25 DEG C, shaking speed is under the condition of 150r / min, carry out fermentation culture after 48.2h on solid slant culture, the fermented liquid is centrifuged (3000rPmin) After 10 minutes, the supernatant was discarded, the precipitate was washed with water, and then centrifuged again. The obtained yeast sludge was dried and weighed, and post-treatments such as concentration and crystallization were performed to obtain β-carotene with a yield of 368 μg / g.

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Abstract

The invention relates to a viscous red torulin for beta- carotene production and the preparing method. The invention provides a viscous Rhodotorula mucilaginosa)CGMCC No.1536 for high- production of beta- carotene. The method comprises: activating viscous red torulin, fermentating for a certain of time, centrifuging and getting bacteria, disintegrating bacteria cell with thermal acid method, adding mixing liquid of petroleum ether and acetone with the proportion being 1: 1, oscillating at 28 Deg. C, extracting for one hour, and getting beta- carotene coarse extract. The invention is characterized by high productivity of beta- carotene, generally reaching 389 ug / g, natural product of beta- carotene, no artificial colouring matter, and wide application of food and other industries.

Description

field of invention [0001] The invention relates to the field of microorganisms and microbial fermentation. Specifically, the present invention relates to a Rhodotorula yeast strain producing β-carotene, β-carotene and a production method thereof. Background technique [0002] β-carotene is an important natural pigment in plants, algae, fungi and other organisms, but it cannot be synthesized in humans and animals and must be ingested from the outside. At present, it is believed that β-carotene has anti-oxidation, anti-tumor, anti-aging effects, such as improving immunity for AIDS patients. In addition, β-carotene is the precursor of vitamin A, has the physiological activity of vitamin A, and can treat upper body cell keratinization, dry eye disease, and night blindness caused by vitamin A deficiency. (Yang Ning, Research Progress on Production of β-Carotene by Fermentation, Food Research and Development, 2004, 25(3): 19-21.) Abbreviation: FAO) and the World Health Organiza...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P23/00C07C403/24C12R1/88
Inventor 艾尔肯·热合曼杨艳艳易霞
Owner XINJIANG UNIVERSITY
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