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Colloid selenium test paper for detecting pulpy kidney, and type of pathogenesis bacteria

A technology of Clostridium welchii and Clostridium welchii, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of good specificity, firm combination, and stable reagents

Inactive Publication Date: 2007-07-25
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, there is no diagnostic test paper for Clostridium welchii disease based on colloidal selenium chromatography at home and abroad, including literature reports and invention patents.

Method used

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  • Colloid selenium test paper for detecting pulpy kidney, and type of pathogenesis bacteria

Examples

Experimental program
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Effect test

Embodiment

[0017] Example (1) Extraction of antigen

[0018] The extraction of the antigen Clostridium weischii alpha toxin has been carried out in two stages: crude extraction and refined extraction.

[0019] Crude extraction: Inoculate the type A Clostridium welschii strain in semi-solid blister meat medium, culture at 37°C for 10 hours, inoculate 0.5% in semi-solid blister meat medium, incubate at 37°C for 6 hours, sterilize with EKS filter plate After filtration, the filtrate is crude alpha toxin.

[0020] Refined extraction: the crude alpha toxin (culture filtrate) was salted out with 70% saturated ammonium sulfate for 30 min, centrifuged at 10000 rpm for 15 min, and the supernatant was discarded; the precipitate was suspended in an equivalent amount of physiological saline solution, and salted out with 20% saturated ammonium sulfate for 30 min at 10000 rpm Centrifuge the supernatant for 15 minutes, then salt out the supernatant with 60% saturated ammonium sulfate for 30 minutes, centri...

Embodiment ( 4

[0070] Example (4) Selenium-labeled monoclonal antibody

[0071] The process of selenium-labeled Clostridium weischii α toxin monoclonal antibody 1 is as follows:

[0072] 1. Preparation of Monoclonal Antibody 1 Solution of Clostridium Wechleri ​​α Toxin to be Labeled

[0073] Preliminarily dialyze the Clostridium velichia α-toxin monoclonal antibody 1 in a 0.005mol / L pH7.0 NaCl solution at 4°C overnight to remove excess salt ions, then centrifuge at 3500rpm at 4°C for 1h to remove the polymer, and use 0.01 PBS for precipitation The buffer (pH8.0) is diluted to 1mg / ml for later use.

[0074] 2. Preparation of colloidal selenium solution to be labeled

[0075] First take a certain amount of glucosan with a concentration of 0.800‰, and a concentration of 1.00×10 -3 mol / L H 2 SeO 3 Place the solution in a 10mL colorimetric tube, mix well and let it stand for a while, add a certain amount of concentration to 5.00×10 -3 mol / L ascorbic acid (Vc) solution, added H2 SeO 3 The ratio of the...

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Abstract

A disease germ type of colloid Se test paper for testing Clostridium welchii disease consists of plastic base plate, Se labeled pad formed by glass cellulose film with detection region and control region, sampling-adding film, water absorption paper and reaction film formed by nitric acid cellulose film with detection region and control region.

Description

1. Technical Field [0001] The invention relates to a selenium gel detection test paper for Clostridium welschii disease and its pathogenic bacteria type, in particular to a selenium gel test paper that can quickly detect the α-toxin of Clostridium wischii in a sample and identify the pathogenic bacteria of Clostridium wischii Type test strips can be used in veterinary clinics, animal husbandry, food safety, public health, water quality monitoring and related fields. 2. Background technology [0002] Clostridium Welchii (Clostridium Welchii), also known as Clostridium perfringens (Clostridium perfringens), is widely distributed in nature, is a typical conditional pathogenic bacteria, and is also a member of the normal flora of animal intestines. It can cause various animal necrotizing enteritis, enterotoxemia, traumatic gangrene of humans and animals, and human food poisoning. It is one of the main pathogens of "sudden death" of livestock in our country in recent years, and poses ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/569G01N33/558G01N33/544
Inventor 李红玉晏磊何丽娟陈瑜
Owner LANZHOU UNIVERSITY
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