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Method of culturing and extracting veralkaol using polygonum cuspidatum produced verakaol endogenic bacteria

A technology of endophytic bacteria of Polygonum cuspidatum and endophytic bacteria of Polygonum cuspidatum is applied in the directions of chemical instruments and methods, bacteria, fungi, etc., which can solve the problems of unseen microbial fermentation and extraction, complicated process operation, low quality products, etc., and achieves operability. Strong, simple extraction and detection process, less environmental pollution

Inactive Publication Date: 2008-11-26
曹庸
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But in general, this type of method has complicated process operation, long cycle and low quality products
[0004] At present, resveratrol is extracted from plants at home and abroad, and there is no report on the use of microbial fermentation to extract

Method used

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  • Method of culturing and extracting veralkaol using polygonum cuspidatum produced verakaol endogenic bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Polygonum cuspidatum endophytic fungus culture and method for extracting resveratrol, the specific steps are:

[0027] (1) Take 200g of potatoes, peel and boil for 30min, filter, dilute the filtrate to 1000mL with distilled water, and sterilize at 121°C for 20min to prepare potato culture solution;

[0028] (2), the endophytic fungi of Polygonum cuspidatum producing resveratrol are cultivated in potato nutrient solution, and the culture conditions are: 20g glucose / L potato nutrient solution, 2g ammonium nitrate / L potato nutrient solution, the culture temperature is 26 ℃, naturally The pH value and medium loading volume are 100mL / 250mL, the inoculum volume is 1 / 10 of the medium loading volume, 0.5g calcium chloride / L potato culture solution, 0.5g ferric chloride / L potato culture solution, 80r / min- 100r / min oscillation speed.

[0029] (3) After taking out the bacterial solution, homogenize it at 100r / min for 15 minutes to break the cell wall (you can add quartz sand as a...

Embodiment 2

[0035] Polygonum cuspidatum endophytic bacteria culture and method for extracting resveratrol, the specific steps are:

[0036] (1) Take 3 g of beef extract, 10 g of peptone, and 5 g of sodium chloride, mix them, dilute to 1000 mL with distilled water, and sterilize at 121° C. for 20 minutes to prepare beef extract peptone culture solution;

[0037] (2), the endophytic bacteria of Polygonum cuspidatum producing resveratrol are cultivated in beef extract peptone culture solution, the culture conditions are: 20g glucose / L beef extract peptone culture solution, 2g ammonium nitrate / L beef extract peptone culture solution, culture The temperature is 35°C, the pH value is 7.2, and the volume of the medium is 100mL / 250mL. The inoculum volume is 1 / 10 of the volume of the medium, 0.5g calcium chloride / L beef extract peptone culture solution and 0.5g magnesium chloride / L beef extract peptone Culture medium, 80r / min-100r / min shaking speed.

[0038] (3) After taking out the bacterial sol...

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Abstract

The present invention provides a method for extracting resveratrol by utilizing culture of polygonaceae endogenic fungi. Said method includes the following steps: (1), using potato to prepare potato culture solution; (2), using glucose as carbon source, using ammonium nitrate as nitrogen source, culturing polygonaceae endogenic fungi or bacteria in culture solution; (3), after the fungous liquid is taken out, homogenizing for 15 min at 100 r / min to make its cell wall be broken, then using ultrasonic wave to make treatment for 30 min; (4), using ethyl acetate to make extraction for 12 h, standing still and layer separation; and (5), collecting extract, concentrating extract and drying extract so as to obtain the resveratrol.

Description

technical field [0001] The invention relates to a microorganism culture technology and a method for extracting effective components of secondary metabolites thereof, in particular to a method for extracting resveratrol by cultivating endophytic bacteria of Polygonum cuspidatum. Background technique [0002] The resveratrol-producing Polygonum cuspidatum endophyte is derived from the Polygonum cuspidatum Siebet Zucc plant, and the endophytic fungus is identified as the genus Penicillium, and the endophytic bacteria is the genus G bacillus Pseudomonas. Polygonum cuspidatum is a small shrub of the genus Polygonaceae, which is a traditional Chinese medicine in my country. Modern pharmaceutical research has proved that its resveratrol (Resveratrol) (3,4',5 trihydroxystilbene) has a variety of pharmacological effects, mainly in anticancer and treatment of cardiovascular disease. [0003] A new search found that domestic and foreign literature and patents on "extracting resveratrol...

Claims

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Application Information

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IPC IPC(8): C07C37/70C07C39/21C12N1/14C12N1/20
Inventor 曹庸
Owner 曹庸
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