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Incubation method for gynogenesis of white crucian

A breeding method and gynogenesis technology, applied in the field of fish gynogenesis, can solve the problems of stress resistance and unsatisfactory meat quality

Active Publication Date: 2008-04-23
HUNAN NORMAL UNIVERSITY
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Problems solved by technology

Japanese white crucian carp (Carassius auratus cuvieri) belongs to Cyprinidae and Cyprinidae. It is one of the fastest-growing diploid crucian carp (2n=100) among crucian carp, and is often used as one of the parents of cross breeding. , but it is still not ideal in terms of stress resistance and meat quality; and Megalobrama amblycephala is a diploid fish (2n=48) belonging to Cyprinidae and Cyprinidae. Compared with Japanese white crucian carp, it belongs to a different species. Subfamily of fish, but its meat is tender

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  • Incubation method for gynogenesis of white crucian
  • Incubation method for gynogenesis of white crucian

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Embodiment Construction

[0009] The invention utilizes the sperm of bream inactivated by ultraviolet rays to activate the eggs of Japanese white crucian carp. Squeeze out the semen of male bream that has reached sexual maturity into a petri dish, dilute the semen with Hank's solution, the ratio of Hank's solution to semen is 4:1, distribute the diluted semen in a thin layer in the petri dish, and put the semen Put the petri dish on a shaker with an ice pack (rotation speed is 30-40 rpm), and place it under two UV lamps with a power of 15 watts for irradiation treatment. The distance between the UV lamp and the semen is 10-12 cm , and the irradiation time was 30 minutes. The formula of Hank's solution is as follows: KCL 0.40g, NaCL 8.00g, NaHCO 3 0.35g, KH 2 PO 4 0.06g, Na 2 HPO 4 ·7H 2 O 0.09g, Na 2 HPO 4 12H 2 O 0.10g, MgSO 4 ·7H 2 O 0.10g, MgCl 2 ·6H 2 O 0.10g, CaCl 2 0.14g, Glucose 1.00g; add distilled water to make up to 1 liter.

[0010] The irradiated semen was mixed with the m...

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Abstract

The present invention relates to gynogenesis technology of fishes. It makes the triangular bream sperm inactivated by UV ray be combined with ovum of Japanese white crusian carp, the activated ovum is undergone the process of cold shock treatment, and the gynogenetic Japanese white crusian carp can be hatched. Said gynogenetic Japanese white crusian carp not only is quick in growth speed, but also its genetic purity and adverse resistance are raised, and its flesh quality also is improved.

Description

technical field [0001] The invention relates to the cultivation of fish, in particular to the gynogenesis of fish. Background technique [0002] Gynogenetic technology plays an important role in fish purification and breeding, research on fish sex determination and fish parthenoculture. There have been many reports on fish gynogenesis at home and abroad, such as carp, grass carp, silver carp, rainbow trout, tilapia, red crucian carp, and white bass. Generally, the breeding of gynogenetic fish is to use fish haploid eggs to be activated by sperm inactivated with genetic material, and then undergo chromosome doubling treatment to form diploid offspring that mainly rely on the genetic material of eggs to develop. The sperm used to activate eggs is generally heterogeneous sperm. Although the heterogeneous sperm has been inactivated, there is a "heterosperm effect", which may have a good effect on gynogenetic offspring. In fish purification and breeding, it is generally believe...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 刘少军刘筠周工健罗凯坤孙远东张纯
Owner HUNAN NORMAL UNIVERSITY
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