Recombinant mumps virus vaccine

a technology of mumps virus and vaccine, which is applied in the field of recombinant mumps virus vaccine, can solve the problems of poor record of generating safe vaccines, high infection velocity, and time-consuming process

Inactive Publication Date: 2017-08-17
UNIV OF GEORGIA RES FOUND INC
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention is about a new isolated nucleotide sequence that includes the full length RNA genome of a mumps virus. This nucleotide sequence can be used to create a new mumps virus vaccine that is safe and effective. The nucleotide sequence can also be used to create a new recombinant mumps virus that can be used for research and development purposes. The invention also includes a method for inducing an immune response to mumps virus in a subject and a method for vaccinating a subject against mumps. The new nucleotide sequence is a valuable tool for research and development of new mumps virus vaccines.

Problems solved by technology

While definitive causes for these recent outbreaks are not known, possible reasons (not mutually exclusive) for these outbreaks include waning immunity, high velocity of infection, and vaccine failure due to emerging of a new mumps virus strain.
The current vaccine Jeryl Lynn (JL) is based in MuV genotype A, while recent outbreaks have been caused by genotype G. It is possible that vaccine generated immunity based on strain A is ineffective in preventing infection of strain G, leading to the outbreak.
This is a time consuming process and a strategy with a poor record of generating safe vaccines.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Recombinant mumps virus vaccine
  • Recombinant mumps virus vaccine
  • Recombinant mumps virus vaccine

Examples

Experimental program
Comparison scheme
Effect test

example 1

Rescue of Wild-Type Mumps Virus from a Strain Associated with Recent Outbreaks Defines Role of the SH ORF in the Pathogenesis of Mumps Virus

[0092]With this example, the complete genome of a representative strain from the epidemic (MuV-IA) was sequenced. MuV-IA is a member of genotype G, the same genotype of MuV that was associated with the outbreak in the UK in 2004-2005. A reverse genetics system was constructed for MuV-IA (rMuV-IA) and used to rescue a virus lacking the open reading frame (ORF) of the SH gene (rMuVΔSH). rMuVΔSH infection in L929 cells induced increased NF-κB activation, TNF-α production and apoptosis compared to rMuV-IA. rMuVΔSH was attenuated in an animal model. These results indicated that the SH ORF of MuV plays a significant role in interfering with TNF-α signaling and viral pathogenesis during virus infection.

Results

[0093]Sequence of the complete genome of MuV-IA. To better understand the genetic characteristics of viruses associated with recent outbreaks in ...

example 2

The V Protein of Mumps Virus Plays a Critical Role in Pathogenesis

[0126]Mumps virus (MuV) causes an acute infection in humans characterized by a wide array of symptoms ranging from relatively mild manifestations, such as parotitis, to more-severe complications, such as meningitis and encephalitis. Widespread mumps vaccination has reduced mumps incidence dramatically; however, outbreaks still occur in vaccinated populations. The V protein of MuV, when expressed in cell culture, blocks interferon (IFN) expression and signaling and interleukin-6 (IL-6) signaling. In this example, a recombinant MuV incapable of expressing the V protein (rMuVΔV) was generated. The rescued MuV was derived from a clinical wild-type isolate from a recent outbreak in the United States (MuVIowa / US / 06, G genotype). Analysis of the virus confirmed the roles of V protein in blocking IFN expression and signaling and IL-6 signaling. It was also found that the rMuVIowa / US / 06 ΔV virus induced high levels of IL-6 exp...

example 3

Immunogenicity of MuVΔSH and MuVΔV in Mice

[0157]The immunogenicity of rMuVΔSH and rMuVΔV in mice was determined and MuV-specific immune responses measured. Mice in a group of 10 were inoculated with PBS, or 106 pfu of MuV, rMuVΔV or rMuVΔSH intranasally. At 21 days post inoculation, blood samples from the mice were collected. Titers of anti-MuV antibodies in the sera were measured using ELISA. The 96-well plates for ELISA were coated with purified MuV virion. P values for MuV and rMuVΔSH, MuV and MuVΔV at highest dilution and lowest dilution of sera were lower than 0.05. The results are shown in FIG. 18.

[0158]Further humoral immunity (antibody) analysis will include a determination of anti-MuV antibodies in bronchoalveolar lavage (BAL), as measured by MuV-specific ELISA. In ELISA assays, the isotypes (IgA, IgG1, IgG2a, IgG2b, and IgG3) of the antibodies will also be determined using appropriate secondary antibodies. MuV-specific antibody titers will also be measured by virus. Neutra...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention provides the complete genomic sequence of the epidemic mumps virus (MuV) strain MuVIowa / US / 06. Further, a reverse genetics system was constructed and used to rescue recombinant viral constructs that are attenuated compared to rMuVIowa / US / 06 and JL vaccine viruses. Such constructs include viral constructs lacking the open reading frame (ORF) of the SH gene (rMuVΔSH) and / or incapable of expressing the V protein (rMuVΔV).

Description

CONTINUING APPLICATION DATA[0001]This application is a continuation of U.S. patent application Ser. No. 14 / 001,228, filed Sep. 25, 2013, which is the §371 U.S. National Stage of International Application No. PCT / US2012 / 026436, filed 24 Feb. 2012, which claims the benefit of U.S. Provisional Application Ser. No. 61 / 446,619, filed Feb. 25, 2011, and U.S. Provisional Application Ser. No. 61 / 529,981, filed Sep. 1, 2011, each of which is incorporated by reference herein.SEQUENCE LISTING[0002]This application contains a Sequence Listing electronically submitted via EFS-Web to the United States Patent and Trademark Office as an ASCII text file entitled “235-01770102 sequencelisting_ST25.txt” having a size of 44 kilobytes and created on Apr. 24, 2017. The information contained in the Sequence Listing is incorporated by reference herein.GOVERNMENT FUNDING[0003]This invention was made with government support under Grant No. K02AI065795, awarded by the National Institutes of Health. The Govern...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(United States)
IPC IPC(8): A61K39/165C12N7/00A61K39/00
CPCA61K39/165C12N7/00A61K2039/5254C12N2760/18743C12N2760/18721C12N2760/18734C12N2760/18762A61K2039/5256A61K39/12A61K39/155A61K2039/543A61P27/16A61P29/00A61P31/00A61P31/12A61P31/14C07K16/1027C07K16/241C07K2317/34C12N2760/18534
Inventor HE, BIAO
Owner UNIV OF GEORGIA RES FOUND INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap