Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Substituted tetrahydroisochinolines as MMP inhibitors, related production method and use as medicine

a technology of mmp inhibitors and substituted tetrahydroisochinolines, which is applied in the direction of antibacterial agents, drug compositions, immunological disorders, etc., can solve the problems of unstable angina pectoris, unstable myocardial infarction or stroke, and plaque instabilities

Inactive Publication Date: 2008-04-17
SANOFI SA
View PDF1 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0024] where optionally independently of one another one or more H atoms in (C1-C6)alkyl, (C1-C6)alkylene, (C1-C4)alkyl, (C1

Problems solved by technology

Moreover, matrix degradation by MMP may cause plaque instabilities or even ruptures, possibly leading to the signs and symptoms of atherosclerosis, unstable angina pectoris, myocardial infarction or stroke (E. J. M. Creemers et al, Circulation Res. 2001, 89, 201-210).
Elevated MMP activity during plaque formation and plaque instability is caused by increased cytokine- and growth factor-stimulated gene transcription, increased zymogen activation and an imbalance in the MMP-TIMP ratio (tissue inhibitors of metalloproteases).

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Substituted tetrahydroisochinolines as MMP inhibitors, related production method and use as medicine
  • Substituted tetrahydroisochinolines as MMP inhibitors, related production method and use as medicine
  • Substituted tetrahydroisochinolines as MMP inhibitors, related production method and use as medicine

Examples

Experimental program
Comparison scheme
Effect test

example 1

Preparation of Exemplary Compounds

Exemplary compounds 3 and 4:

3: 2-[4-(4-Fluorophenoxy)benzenesulfonyl]-6-[3-(4-methylpiperazin-1-yl)propoxy]-1,2,3,4-tetrahydroisoquinoline-1-carboxylic acid hydroxamide;

4: 2-[4-(4-Fluorophenoxy)benzenesulfonyl]-6-[3-(4-methylpiperazin-1-yl)propoxy]-1,2,3,4-tetrahydroisoquinoline-1-carboxylic acid;

3A: 4-Fluorophenoxybenzenesulfonyl chloride;

[0147] 16.1 g (0.138 mol) of chlorosulfonic acid are slowly added dropwise to a solution of 13.05 g of 4-fluorophenoxybenzene (0.069 mol) cooled to 0° C. After the addition is complete, the mixture is stirred at room temperature for a further 4 hours. For work up, 50 ml of dichloromethane are added, and the mixture is washed once with H2O. The aqueous phase is separated off and extracted twice more with 50 ml of dichloromethane each time. The combined organic phases are dried with MgSO4 and concentrated, resulting in the title compound as a colorless solid. Yield: 11.03 g; 60%.

3B: Methyl 2-[4-(4-fluoropheno...

example 2

Catalytic Inhibition of Gelatinase B (MMP-9) in Human Serum by Synthetic MMP Inhibitors

[0190] Freshly isolated human serum was shock-frozen in the desired volume in liquid N2 and then stored at −80° C. For the catalytic inhibition of MMP-9 in human serum, the appropriate parts by volume of human serum were thawed at room temperature. 100 parts by volume of undiluted, freshly thawed human serum were mixed with 1 part by volume of test substance, thoroughly mixed and incubated at 37° C. for 1 h. The test substances were dissolved in 100% dimethyl sulfoxide, and a 10 mM stock solution was prepared. For dose-effect plots, the test substances were tested in final concentrations of from 200 μM to 0.001 μM for their catalytic inhibition of MMP-9 in human serum. The final concentration of DMSO in the reaction solution was 1% in each case.

[0191] Active MMP-9 from the reaction solutions described above was determined with the aid of a MMP-9 activity assay system (Amersham Pharmacia Biotech ...

example 3

Determination of the Solubility by Means of a CLND (Chemo Luminescence Nitrogen Detector)

[0205] For the thermodynamic solubility measurement, 1 mg of solid substance is taken up in 250 μl of PBS buffer (sodium phosphate 10 mM; pH 7.4; 0.9% NaCl isotonic salt concentration) and mixed, and the suspension is shaken at room temperature for 16 hours. The solution is then centrifuged at 14 000 rpm for 5 minutes. The solution is removed from the pellet and filtered through a syringe filter (0.45 μm pore size). The presence of a pellet is checked in order to ensure that a saturated solution was present. The filtered clear solution is analyzed by HPLC.

[0206] The dissolved amount of substance is quantified using a generic chromatographic method:

[0207] Either a calibration line derived from DMSO stock solutions (1-500 μM) of the appropriate substance is used to quantify the corresponding signal in the UV (254 nm) chromatogram of the solubility sample, or the quantification is undertaken via...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Forceaaaaaaaaaa
Forceaaaaaaaaaa
Forceaaaaaaaaaa
Login to View More

Abstract

The present invention is directed to a compound of formula (1), wherein R1, R2, R3, R4, A, L and n are as defined herein, its pharmaceutical composition, preparation and uses as a MMP inhibitor.

Description

[0001] This application is a Continuation of International Application No. PCT / EP2006 / 002440, filed Mar. 17, 2006.BACKGROUND OF THE INVENTION [0002] In diseases such as osteoarthritis and rheumatism there is destruction of the joint caused in particular by the proteolytic breakdown of collagen by collagenases. Collagenases belong to the superfamily of metalloproteinases (MP) or matrix metalloproteinases (MMP or MMPs). The MMPs form a group of Zn-dependent enzymes involved in the biodegradation of the extracellular matrix (D. Yip et al., Investigational New Drugs 1999, 17, 387-399 and Michaelides et al., Current Pharmaceutical Design 1999, 5, 787-819). These MMPs are capable in particular of breaking down fibrillary and non-fibrillary collagen, and proteoglycans, both of which represent important matrix constituents. MMPs are involved in processes of wound healing, of tumor invasion, metastasis migration and in angiogenesis, multiple sclerosis and heart failure (Michaelides et al., i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/472A61K31/496A61K31/5377A61P19/00A61P29/00A61P35/00A61P9/00C07D401/02C07D413/02
CPCC07D403/12C07D217/26A61P1/02A61P1/04A61P1/14A61P17/02A61P19/00A61P19/02A61P21/00A61P29/00A61P31/04A61P35/00A61P35/04A61P37/02A61P43/00A61P9/00A61P9/04A61P9/10
Inventor HOFMEISTER, ARMINSCHUDOK, MANFREDMATTER, HANSBREITSCHOPF, KRISTINUGOLINI, ANTONIO
Owner SANOFI SA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com