Stimulation of the synthesis of the activity of an isoform of lysyl oxidase-like LOXL for stimulating the formation of elastic fibres

Abstract

The invention relates to the stimulation of the synthesis and of the activity of an isoform of lysyl oxidase, and more particularly of the LOXL (Iysyl oxidase-like) isoform. The invention relates notably to a method of identifying an active principle which stimulates the formation of elastic fibers. The aim of the invention is mainly to provide such a method of identification so as to provide compositions which enable stimulating the formation of elastic fibers.

Description

[0001] The invention relates to the stimulation of the activity of an isoform of lysyl oxidase, and more particularly of the LOXL (lysyl oxidase-like) isoform.STATE OF THE ART[0002] The properties of resistance and of elasticity of the skin and of the mucous membranes are essentially defined by the collagen fibers and elastin fibers of the dermis. Elastin is a protein which makes up the elastic fibers by combining itself with other molecules such as fibrillins and MAGPs (Microfibrillar Associated Glycoproteins).[0003] The elastic fibers are formed of elastin deposited on the microfibrils. Elastin is synthesized in the form of soluble tropoelastin which acquires its physico-chemical properties (insolubility, elasticity) after the intra- and inter-molecular cross-linking of it by a lysyl oxidase, and its deposit on the microfibrils. The elastic fibers are responsible for the elastic property of the organs which contain them (vessels, pulmonary parenchyma, elastic cartilages, skin . . ...

AI Technical Summary

Benefits of technology

[0032] Advantageously, the use described above is carried out for inducing a neo-elastogenesis of the tissues, and notably for stimulating the elasticity of the tissues thus obtained, and for reducing skin wrinkles.

[0033] Advantageously, the use described above is carried out for combating against the loosening of the tissues, notably when the loosening of the tissues is observed during ageing or during solar exposures, for densifying the extracellular matrix, for firming up the subcutaneous tissues, for reducing skin wrinkles, for exerting an anti-wrinkles effect, for improving the quality of scar tissue and the appearance of scars, notably dystrophic and keloid scars, or for combating against stretch marks.

[0044] Advantageously, the cosmetic care is selected from the group consisting of combating against the loosening of the tissues, notably when the loosening of the tissues is observed during ageing or during solar exposures, densifying the extracellular matrix, firming up the subcutaneous tissues, reducing skin wrinkles, anti-wrinkles effects, improving the quality of scar tissue and the appearance of scars, notably dystrophic and keloid scars, and combating against stretch marks.

[0090] Advantageously, this method of treatment enables performing a treatment selected from combating against the loosening of the tissues, notably when the loosening of the tissues is observed during ageing or during solar exposures, densifying the extracellular matrix, firming up the subcutaneous tissues, reducing skin wrinkles, anti-wrinkles effects, improving the quality of scar tissue and the appearance of scars, notably of dystrophic scars, in particular dystrophic scars, and keloid scars, and combating against stretch marks.

Problems solved by technology

Similarly, in scars, the network of elastic fibers does not form correctly.

The prior art does not enable providing criteria which enable evaluating the impact of an active principle in dermato-cosmetology on a functional neo-elastogenesis.

In this context, it is also very difficult to obtain objective criteria enabling the impact of these actives to be judged.

Furthermore, at the present time, animal experimentation is forbidden in cosmetics in Europe and human experimentation is ethically disputed.

It is therefore unacceptable to the inventors to carry out a screening method, for cosmetic applications, which makes use of animals or human beings.

However, those studies did not enable developing a method of identifying active principles which enable stimulating the formation of functional elastic fibers.

The prior art does not therefore enable providing active principles which enable stimulating the formation of functional elastic fibers.

Furthermore, the prior art does not enable a dynamic tracking of the zone of expression of the isoform of the lysyl oxidase LOXL, notably due to the fact that the methods provided by the prior art are imprecise.

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