Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Guanine one-pot synthesis method

A synthesis method and guanine technology are applied in the field of one-pot synthesis of guanine, can solve problems such as long synthesis routes, and achieve the effects of convenient operation and high yield

Inactive Publication Date: 2007-05-23
SHANGHAI INST OF PHARMA IND
View PDF6 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method must use prepared and purified DAHNP as a raw material to synthesize guanine, and the synthesis of DAHNP from aliphatic chain compounds also requires several steps of reaction, and the synthetic route is also relatively long

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Guanine one-pot synthesis method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Add 400mL of absolute ethanol and 18.0g (0.783mol) of sodium metal into a 2L four-neck flask, stir, and add 110.0g (0.898mol) of guanidine nitrate after it is completely dissolved, heat to 70°C and stir for 0.8 hours. 56.0 g (0.486 mol) of ethyl cyanoacetate was added dropwise, heated to 70° C. (range 50° C. to reflux) and reacted for 2 hours, and the ethanol was evaporated to dryness under reduced pressure. Add 600ml of formamide to dissolve, and cool to 4°C in ice water. Add 40.0 g (0.580 mol) of sodium nitrite, add dropwise a mixed solution of 100 ml formamide and 100 ml 98% formic acid, and control the temperature below 5°C. After dropping, react at 4°C for 2.5 hours. Heat to 100°C, add 20.0 g (0.192 mol) of sodium bisulfite, and react at 100°C for 1.5 hours. Reaction was carried out at 180° C. for another 2 hours. Cool, filter and wash with water several times. After drying, 147g of brown solid was obtained.

[0027] Add 2000 mL of 2.75N hydrochloric acid to t...

Embodiment 2

[0029]Add 200 mL of anhydrous methanol and 16 g (0.70 mol) of sodium metal into a 2L four-neck flask, stir, and add 86.0 g (0.702 mol) of guanidine nitrate after it is completely dissolved, heat to 50°C, and stir for 0.5 hours. Add 56.0 g (0.486 mol) of ethyl cyanoacetate dropwise, heat to 50° C., react for 1 hour, and evaporate methanol to dryness under reduced pressure. Add 300 mL of formamide to dissolve, and cool to 4°C in ice water. Add 37g (0.535mol) of sodium nitrite, add dropwise a mixed solution of 100ml formamide and 80ml 98% formic acid, control the temperature not to exceed 10°C, and react at 10°C for 2 hours after dropping. Heat to 80°C, add 30.0 g (0.288 mol) of sodium bisulfite, and react at 80°C for 1 hour. Reaction was carried out at 150° C. for another 4 hours. Cool, filter and wash with water several times. After drying, 140.5 g of brown solid was obtained.

[0030] Add 1500 mL of 2N hydrochloric acid to the above solid, decolorize it with 10 g of activa...

Embodiment 3

[0032] Add 1000 mL of isopropanol and 76 g (1.95 mol) of potassium metal into a 2L four-neck flask, stir, and add 218.7 g (1.944 mol) of guanidine nitrate after it is completely dissolved, heat to reflux temperature, and stir for 2 hours. 112.0 g (0.972 mol) of ethyl cyanoacetate was added dropwise, heated to reflux temperature, reacted for 10 hours, and evaporated to dryness of isopropanol under reduced pressure. Add 1000mL of formamide to dissolve, cool to 4°C in ice water, add 100.5g (1.458mol) of sodium nitrite, add dropwise a mixed solution of 100ml of formamide and 110ml of 98% formic acid, and control the temperature below 5°C. After dropping, react at 4°C for 10 hours. Heat to 180°C, add 40.0 g (0.384 mol) of sodium bisulfite, and react at 180°C for 5 hours. 200°C for another 10 hours. Cool, filter and wash with water several times. After drying, 283 g of brown solid was obtained.

[0033] The above solid was recrystallized by adding 3000 mL of 3N hydrochloric acid...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a synthesis method for guanine, which includes the following steps: carrying out a cyclization reaction of guanidine nitrate and ethyl cyanoacrylate to obtain the compound of 2, 4-diamino-6-hydroxypyrimidine in formamide solvent, adding sodium nitrite, formamide and concentrated formic acid in turn, and carrying out nitrosation and reduction reaction with reducing agent and cyclization reaction to obtain the final product. The invention is the use of ''one-pot'', and the raw materials are fat chain ethyl cyanoacrylate and guanidine nitrate, which are directly from market, cheap and easy to get, without need of separation and purification of pyrimidine intermediates. Guanine is prepared by one step through effective control of reaction conditions, feeding sequence and ratio. The product is purified through adjusting pH, which has high yield and is easy to operate.

Description

technical field [0001] The invention relates to a synthesis method of guanine, in particular to a one-pot synthesis method of guanine. Background technique [0002] Guanine is an important substance for the synthesis of antiviral drugs. For example, the synthesis of antiviral drugs aciclovir (ACV) and ganaciclovir (GCV) antiviral drugs are all synthesized from guanine as a starting material. Its chemical name is: 2-aminopurine, and its molecular formula is: C 5 h 5 N 5 O, the structural formula is shown in formula 6 in the synthetic route below. Due to the extremely poor water solubility and ester solubility of guanine, although guanine has long been chemically synthesized, it has always been a difficult problem to obtain a product whose nitrogen content meets the requirements of elemental analysis. Simultaneously, the synthesis of guanine from aliphatic chain compounds (such as guanidine nitrate and ethyl cyanoacetate) mostly requires multi-step reactions, and the synt...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07D473/18
Inventor 肖旭华马维勇
Owner SHANGHAI INST OF PHARMA IND
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com