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Small molecule bioactive peptide, preparation method, composition and application thereof

A bioactive peptide and molecular biology technology, applied in the direction of drug combination, application, food preparation, etc., can solve the problems of limited source of raw materials, difficulty in extraction, and restriction of development, so as to improve the body's immune function and promote the healing of body tissue wounds Effect

Inactive Publication Date: 2007-02-28
SHANGHAI MIYANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] First, it is extracted from animal and plant tissues and organs. Due to the limited source of raw materials and the difficulty of extraction, the product cost is high, and it is limited to the production of certain peptide drugs with special curative effects
[0009] Second, through the synthesis of chemical methods (liquid phase method or solid phase method), active peptides can be synthesized arbitrarily according to people's wishes, but this method has problems such as high cost and many side reactants, which seriously restrict its development. Synthetic methods are limited to theoretical research on some peptide drugs and certain biologically active peptides
[0011] To sum up, the existing bioactive peptide raw materials mainly use a single milk protein, soybean protein or egg white protein, etc., and the formed peptide structure is relatively simple, and the function is relatively simple

Method used

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  • Small molecule bioactive peptide, preparation method, composition and application thereof
  • Small molecule bioactive peptide, preparation method, composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] The preparation of embodiment 1 small molecule bioactive peptide

[0079] 1. Determination of raw material ratio

[0080] Taking the degree of hydrolysis DH of the substrate protein as an index, papain and neutral protease are 50% compound enzymes, and the amount of enzyme added is 1%, respectively, at pH7. To determine the optimal ratio of raw materials.

[0081] Under the conditions of papain: neutral protease = 50%: 50%, enzyme addition 1%, pH 7, temperature 50°C, and hydrolysis time of 5 hours, taking the degree of hydrolysis DH of the substrate protein as an index, the different protein raw materials Ratio single factor analysis to determine the best ratio of raw materials.

[0082] The weight ratio (calculated by dry matter) of each protein raw material is as follows:

[0083] Substrate 1: soybean protein: whey protein: egg: milk=50%: 20%: 15%: 15%;

[0084] Substrate 2: soybean protein: whey protein: egg: milk=55%: 15%: 10%: 20%;

[0085] Substrate 3: soybea...

Embodiment 2

[0096] Embodiment 2 Manufacture and detection of small molecule bioactive peptide composition

[0097] The small molecule biologically active peptide of the present invention can be prepared in the form of capsules. According to the conventional way of preparing medicine or health product capsules, freeze-dry the small molecular bioactive peptides prepared above, pass through a 100-mesh sieve, and machine-fill No. 0 capsules with a solid carrier, each capsule has a loading capacity of 0.3g, and compression-molded aluminum foil Board, 12 capsules per board. After passing the inspection, it can be packaged as a finished product.

[0098] 1. Physical and chemical index detection

[0099] Three batches of capsule samples prepared above were selected, namely sample 1, sample 2, and sample 3. According to the detection methods stipulated in GB / T5009-2003 and "Pharmacopoeia" 2000 edition, the product, protein, moisture, ash, disintegration time limit, lead, arsenic, mercury and ot...

Embodiment 3

[0109] The toxicity test of embodiment 3 small molecule bioactive peptide composition

[0110] 1. Rat acute oral toxicity test

[0111] 20 SD rats (purchased from the Experimental Animal Center of the Fourth Military Medical University), half female and half male, with a body weight of 180-220g, were fasted overnight for 16 hours before the test, and the bioactive peptide capsules prepared in Example 2 were pressed by 15.0g The dose per kg.bw (equivalent to 750 times the recommended amount for the human body), with a gavage capacity of 1.5mL / 100g / kg.bw, was administered three times (interval 4 hours). Animal performance was then observed for 14 consecutive days. Mix the contents of the capsules with distilled water during intragastric administration to prepare each dosage concentration. The results are shown in Table 8.

[0112] gender

number of animals

(Only)

way

dosage

(g / kg.bw)

Circumstances of death

...

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Abstract

The invention discloses the little molecule biological activity peptide, which is from the mixture of soy protein, lactalbumin, egg and milk. The molecular weight of 90% of the little molecule biological activity peptide is less 5KDa. The invention also provides the preparing method. The invention also provides the composition of biological activity peptide, which comprises the said little molecule biological activity peptide and carriers of pharmacy and foodstuff. The little molecule biological activity peptide can improves the immunity, promote wound healing and enhance health.

Description

technical field [0001] The present invention relates to the field of biologically active peptides, more specifically, the present invention relates to a small molecule biologically active peptide and its preparation method, composition and application. Background technique [0002] Peptides are compounds consisting of two or more amino acids linked by peptide bonds. Bioactive peptide refers to the general term of a class of peptides that can regulate the life activities of biological organisms or have certain physiological activities. The physiological effects of bioactive peptides on the human body can be manifested in the following aspects: ①regulate the secretion of hormones; ②regulate immunity; ③antibacterial; ④anticancer; ⑦ Antioxidant; ⑧ Antihypertensive. Because small molecule active peptides have the above-mentioned physiological functions, they are receiving more and more attention in the fields of medicine, food and animal feed. [0003] Peptides are biologicall...

Claims

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Application Information

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IPC IPC(8): C12P21/02A61K38/03A61K38/01A61K9/08A61K9/10A61K9/16A61K9/20A61K9/48A61K9/00A61P37/04A61P17/02A23L1/29A23J3/34
Inventor 陈五岭曹珉
Owner SHANGHAI MIYANG BIOTECH
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