Hemoglobin chip preparation and electrochemical detecting method thereof

A hemoglobin and electrochemical technology, applied in the direction of material electrochemical variables, biological testing, material inspection products, etc., can solve the problems of high cost, complicated operation, unfavorable promotion and use, etc., and achieve low cost, fast analysis speed, and good water solubility Effect

Inactive Publication Date: 2007-02-07
SOUTHEAST UNIV
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Problems solved by technology

All these methods require special reagents and spectroscopic instruments, and the operation is complicated and costly, which is not conducive to popularization and use.

Method used

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Examples

Experimental program
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Embodiment Construction

[0029] Preparation of Prussian Blue Nanoparticles

[0030] The size and properties of Prussian basket nanoparticles are related to their preparation process. The present invention selects polyvinylpyrrolidone K-30 with a weakly positive charge on the surface as a stabilizer, adds it to a 0.1mol / L potassium hexacyanoferrate solution, and slowly adds an equivalent amount of oxychloride dropwise under stirring. After the iron solution was added dropwise, the stirring was continued for 5 hours to obtain Prussian blue nanoparticles wrapped by polyvinylpyrrolidone.

[0031] Preparation of printed carbon electrodes

[0032] (1) Choose PVC material as the substrate, engrave a track of 1 mm × 3 cm on it, clean it thoroughly with NaOH solution and deionized water, and brush it with silver paste for conductivity after drying.

[0033] (2) Printing graphite ink made of polyvinyl alcohol, ultrafine graphite powder, and cellulose diacetate on the above-mentioned silver paste track to form...

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Abstract

The preparation method for hemoglobin chip comprises: 1) preparing the print carbon electrode; 2) preparing and modifying the Prussian blue nano particle; 3) evaporating and drying the particle to dissolve into 0.1M phosphate buffer solution with pH value as 7.0 and spraying on the electrode surface, drying in icebox for standby. Besides, the electrochemical detection method comprises: 1) optimizing the test condition: a) preparing standard solution; b) selecting detection potential; c) setting pH value for the buffer solution; 2) plotting the normal curve; 3) correcting the curve. This invention is simple and fast with low cost.

Description

technical field [0001] The invention relates to the preparation of a hemoglobin clinical detection chip, in particular, a method for measuring hemoglobin content in blood is established in combination with electrochemical analysis technology. Background technique [0002] Hemoglobin (Hemoglobin) is the respiratory protein in the red blood cells of vertebrates. It is the main substance that transports oxygen in the blood. It plays the role of transporting oxygen and decomposing H 2 o 2 Important activities related to oxygen and energy metabolism, such as transfer of electrons, play a key role in all life activities. The relative molecular mass of hemoglobin is about 67,000, and its molecule has a quaternary structure. It is a tetramer composed of two α and two β polypeptide chains. Each peptide chain is bound to a heme molecule and is close to each other. Form approximately spherical hemoglobin molecules with a diameter of about 55nm. Heme is located in a hydrophobic envir...

Claims

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Application Information

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IPC IPC(8): G01N33/72G01N27/30G01N21/25
Inventor 刘松琴
Owner SOUTHEAST UNIV
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