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Use of short-leave geranium component in preparation of tumor growth and proliferation inhibitor

A kind of geranium brevis, proliferation inhibition technology, applied in the direction of antitumor drugs, medical preparations containing active ingredients, plant raw materials, etc., to achieve good application prospects, curb infinite proliferation ability, reduce the possibility of negative effects Effect

Inactive Publication Date: 2007-02-07
湖北中医学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] At present, no patents on the use of geraniol in the preparation of anticancer drugs have been found in relevant searches. However, the current problem in the field of cancer chemotherapy treatment is to find new low-toxicity and high-efficiency anticancer drug resources

Method used

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  • Use of short-leave geranium component in preparation of tumor growth and proliferation inhibitor
  • Use of short-leave geranium component in preparation of tumor growth and proliferation inhibitor
  • Use of short-leave geranium component in preparation of tumor growth and proliferation inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Toxic effects of geraniol on tumor cells:

[0034] Geranitin is a pure natural product isolated from Goose Bubivora, with a purity greater than 99%. It is diluted with DMEM (Dulbecco's modified Eagle medium, DMEM) culture solution to the required concentration. The toxic effect of geraniin on A375, A431, 95D, MKN45, BEL-7402, LoVo, HL60 cells was determined by rapid colorimetric method of thiazolium blue (MTT). Cells in logarithmic growth phase (10 6 Cells / ml), inoculated in 96-well culture plate, 0.2 ml per well, respectively added geranidin at a set concentration for treatment, 4 wells in parallel for each concentration, added an equal volume of culture medium to the control group, and placed at 37°C , 5% CO 2 Incubate in an incubator with saturated humidity for 1-4 days, add 10 microliters of 5 mg / ml MTT to each well 4 hours before the end of the experiment, and add 0.04 equivalent of dimethyl sulfoxide (DMSO) to each well after the end of the culture, 150 Microli...

Embodiment 2

[0042] Toxic effects of geraniol on normal L02 cells:

[0043] Geranitin brevis is a pure natural product isolated from Goose Bubivora with a purity greater than 99%. It is diluted with DMEM culture solution to the required concentration. The toxic effect of geraniin on L02 cells was determined by rapid colorimetric method of thiazolium blue (MTT). Cells in logarithmic growth phase (10 6 Cells / ml) were inoculated in a 96-well culture plate, 0.2 ml per well, were treated with geraniol at a set concentration, and 4 wells were paralleled for each concentration, and an equal volume of culture solution was added to the control group, and placed at 37°C. 5%CO 2 Cultivate in an incubator with saturated humidity for 1-4 days. Add 10 microliters of 5 mg / ml MTT to each well 4 hours before the end of the experiment. liter, shake for 10 minutes until the MTT reduction product is completely dissolved, use a BioRad 550 microplate reader to measure its absorbance with 550 nm as the experi...

Embodiment 3

[0046] Comparison of the activity of geraniol in inhibiting tumor growth in C57 mice:

[0047] Geranitin brevifolia is a pure natural product isolated from Goose Bubivora, with a purity greater than 99%. Male, two tumor-bearing C57 mice, the solid tumors of the mice were stripped, chopped and ground with acid phosphate buffer (pH 7.4), and filtered through an 80-mesh sieve. Make a cell suspension for later use. Male C57 were randomly divided into groups according to body weight, and tumor cell suspension (1×10 6 Individuals / ml), different drugs were intraperitoneally injected daily at the same time of inoculation, and the negative control was normal saline (PBS). Anticancer drugs were used as positive control, administered for 8 days, then sacrificed, and the solid tumors of the mice were removed and weighed. The inhibition rate was calculated compared with the control. The experimental results of the effect of different concentrations of drugs on the tumor inhibition rate...

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Abstract

The invention discloses the use of Brevlin in preparing suppressing agent against tumor growth and proliferation. Extracorporal and animal in vivo experiment shows that, Brevlin causes very low degree of toxicity to normal cells.

Description

technical field [0001] The present invention relates to the application of brevilin, in particular to the application of brevilin in the preparation of tumor growth and proliferation inhibitors. Background technique [0002] Goose is not herbivorous (Centipeda minima (L.) A.Br.et Aschers.) is a plant of the genus Asteraceae, also known as Centipeda minima, and chicken intestine grass. The fact that geese do not eat grass was first recorded in the "Eat Habit of Materia Medica" in the Southern Tang Dynasty, and it is also recorded in the famous Chinese pharmacopoeia "Compendium of Materia Medica", "The goose does not eat grass can clear the nose, sharpen the seven orifices, spit out wind and phlegm, and stop the nose from falling." . Goose does not eat grass and grows in damp places at an altitude of 300 to 1900 meters. It is mainly produced in Zhejiang, Hubei, Jiangsu, Guangdong and other places in my country. Abroad, it is mainly distributed in ...

Claims

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Application Information

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IPC IPC(8): A61K31/343A61K36/28A61P35/00
Inventor 吴和珍刘建文刘焱文杨艳芳李长龙
Owner 湖北中医学院
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