Ultrasonic process for extracting pine needle total flavone

A technology of flavonoids and pine needles, which is applied in the field of extraction technology of total flavonoids of pine needles, can solve the problems of waste of crude drug resources, long processing cycle, and low extraction rate, and achieve the effects of shortening extraction time, improving utilization rate, and convenient operation

Inactive Publication Date: 2007-01-31
兰州奇正生态健康品有限公司
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the existing pine needle extraction adopts alcohol extraction or water extraction method, which has a low extraction rate, long processing cycle, high cost and low extraction rate, and also wastes crude drug resources.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1, the preparation method of pine needle flavonoid extraction process of the present invention comprises the following process steps:

[0020] ① Wash and dry the dry needles of Pinus massoniana, which is pollution-free on the Qinghai-Tibet Plateau, with drinking water;

[0021] ②At room temperature, add an 80% ethanol solution 8 times the volume of the needles to the needles and soak for 20 hours;

[0022] ③Use 50khz ultrasonic wave, ultrasonic treatment at 100v for 40 minutes, recover the extract, concentrate to a density of 0.8-1.2g / cm3;

[0023] ④ extract the concentrated solution with petroleum ether of 2 times its volume, and collect the extract;

[0024] ⑤ The extract of petroleum ether is extracted once with ethyl acetate of 2 times its volume, and the extract is collected;

[0025] ⑥Concentrate the extract to a density of 1.0-1.6g / cm3, and vacuum freeze-dry to obtain pine needle flavonoids.

Embodiment 2

[0026] Embodiment 2, the preparation method of pine needle flavonoid extraction process of the present invention comprises the following process steps:

[0027] ① Wash and dry the dry needles of Pinus massoniana, which is pollution-free on the Qinghai-Tibet Plateau, with drinking water;

[0028] ②At room temperature, add 60% ethanol solution 10 times the volume of the needles to the needles and soak for 12 hours;

[0029] ③Use a 30khz ultrasonic wave at 150v for 40 minutes; recover the extract and concentrate it to a density of 0.8-1.2g / cm3;

[0030] ④The concentrated solution was repeatedly extracted 4 times with its same volume of sherwood oil, and the extracts were combined;

[0031] ⑤ The extract of petroleum ether is repeatedly extracted 3 times with 3 times the amount of ethyl acetate, and the extracts are combined;

[0032] ⑥Concentrate the extract to a density of 1.0-1.6g / cm3, and vacuum freeze-dry to obtain pine needle flavonoids.

Embodiment 3

[0033] Embodiment 3, the preparation method of pine needle flavonoid extraction process of the present invention comprises the following process steps:

[0034] ① Wash and dry the dry needles of Pinus pine plant Pinus massoniana with drinking water;

[0035] ②At room temperature, add 75% ethanol solution 9 times the volume of the needles to the needles and soak for 16 hours;

[0036] ③Use a 40khz ultrasonic wave at 130v for 25 minutes; recover the extract and concentrate it to a density of 0.8-1.2g / cm3;

[0037] 4. The concentrated solution is repeatedly extracted 4 times with the sherwood oil of 1.5 times its volume, and the extracts are combined;

[0038] 5. the extract of petroleum ether is extracted with its ethyl acetate of the same volume, and the extract is collected;

[0039] ⑥Concentrate the extract to a density of 1.0-1.6g / cm3, and vacuum freeze-dry to obtain pine needle flavonoids.

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Abstract

An ultrasonic technology for extracting general flavone from pine needles includes such steps as drying, washing with drinking water, drying, immersing in alcohol solution, ultrasonic processing, collecting the liquid extract, extracting respectively by petroether and ethyl acetate, collecting extracted liquid, concentrating and vacuum freeze-drying. It has high output rate of usnic acid.

Description

technical field [0001] The invention relates to an extraction process of total flavonoids from pine needles, and also relates to the application of ultrasonic extraction in the extraction of flavonoids. Background technique [0002] Pine needles have been recorded in ancient herbal medicines. They have the effects of dispelling wind and promoting blood circulation, improving eyesight, calming the nerves, detoxifying, drying dampness and relieving pain. They are used to treat hypertension, neurasthenia, rheumatoid arthritis and other diseases. [0003] Show according to research: contain abundant flavonoid glucoside in the pine needle, the injection that is made of pine needle flavonoid injects in vitro to staphylococcus aureus, catarrhal coccus has certain bacteriostatic effect, has cooling effect to the rabbit intraperitoneal injection of fever; Dog intravenous injection (5mg / g), can lower blood pressure and increase coronary flow (33%-36%). It can also increase coronary f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/15A61P9/12A61P25/20A61P19/00A61P19/02
Inventor 雷菊芳
Owner 兰州奇正生态健康品有限公司
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