Preparation of fungi extract and application thereof in resisting HIV

Abstract

Disclosed is an applocation of an abstract of a plant endogenetic fungi fermentation product for antagonizing immunodeficiency virus(HIV). Said fungi is isolated from the roots of Orchidaceae medicinal herb Dendrobium nobile, and is EF1(Epulorhiza sp.); EF1s are fluid fermentation cultured to prepare an abstract of the fermentation product. The invention is provided with simplicity of fermentation art, short period of production, and mass industrialization production. The abstract of the fermentation product is obtained easily, and has a remarkable anti-HIV-1 activity, the acting site of which is after reverse transcription and before HIV RNA expression, with a regulation action of HIV-1 gene expression. An adding action is obtained by a combined use of the said fungi fermentation product and anti-HIV drug AZT, indicated by a drug combination test. The abstract of the fungi fermentation product has an important value in the development of novle anti-HIV drugs, and is hopefule to become a novel anti-HIV drug.

Description

technical field [0001] The invention belongs to the technical field of bioengineering. Specifically, it relates to a preparation technology of an endophytic fungus extract which has anti-human immunodeficiency virus (HIV-1) activity and can regulate HIV-1 gene expression. technical background [0002] Acquired Immunodeficiency Syndrome, also known as AIDS (AIDS), was first reported by the US Centers for Disease Control in 1981. In the mid-1980s, AIDS had developed into a global epidemic, and its pathogen was identified as Human Immunodeficiency Virus (Human Immunodeficiency Virus, HIV). Over the past two decades, research in the field of AIDS pathology and treatment has achieved certain results. In recent years, high-efficiency antiviral treatment has significantly reduced the morbidity and mortality of AIDS. However, there are currently the following problems in the treatment of AIDS: on the one hand, the high variability of HIV makes it easy to produce drug resistance; o...

AI Technical Summary

Problems solved by technology

However, there are currently the following problems in the treatment of AIDS: on the one hand, the high variability of HIV makes it easy to develop drug resistance; on the other hand, chemotherapy drugs have strong toxic and side effects, and most of these drugs are expensive, and many AIDS patients in economically backward countries and regions cannot afford medical expenses, so they cannot receive timely and effective treatment

[0004] Searching for anti-HIV drugs with high efficiency and low toxicity from traditional Chinese medicine has always been a very active field in the development of new drugs at home and abroad, but the large collection of plants may lead to the depletion of plant resources, resulting in the destruction of vegetation and human living environment