Near field enhanced Raman molecular fingerprint spectrum analysis method

Abstract

This invention relates to a near field enhanced Raman molecular fingerprint spectrum analysis method<0}, which comprises an optimized near-field reinforced Raman sample pond, an inverted or vertical microscope, and a Raman spectrometer. The analysis can comprise five steps or less according to request, which comprises mainly: preparing the sample into liquid for direct use, or taking full 2D gel electrophoretic separation; comparing the determined data with database. This invention special fit to active protein constituent analysis and medical research.

Description

technical field [0001] The invention relates to the technical field of biomacromolecule analysis, in particular to a proteomics near-field enhanced Raman molecular fingerprint analysis method. Background technique [0002] In spectroscopic analysis technology, molecular Raman scattering is due to the small scattering cross section (about 10 -29 cm 2 ) applications are very limited. In recent years, the surface-enhanced Raman scattering (SERS) technology can increase the Raman scattering cross section by many orders of magnitude, expanding the application range of Raman analysis. In 2002, Wu Shifa and Wu Guanying applied for the invention patent of "Raman scattering near-field enhanced sample cell with evanescent light for both excitation and reception" (application number: CN021544689). The application of this invention patent can greatly enhance the detection of "hot spots" in the sample cell. Electric field strength and increase the number of "hot spots" in the Raman an...

AI Technical Summary

Problems solved by technology

[0006] (1) It is still difficult to analyze proteins with large and extra large molecular weights;

[0007] (2) Biomass spectrometry methods cannot distinguish isomeric protein molecules;

[0008] (3) It is impossible to maintain the activity of proteome biological samples, and it is not possible to analyze them in situ, and it is often necessary to disassemble the samples for analysis;

[0009] (4) The structural relationship between molecules in the proteome cannot be analyzed;

[0010] (5) The content of some protein molecules in some cells is very small, but the function is very special, and the above method is not competent;

[0011] (6) Proteomics analysis requires a huge amount of data and extremely fast analysis speed, which is still difficult to meet the requirements;

[0012] (7) The preparation technology of extremely high-purity samples is relatively difficult, and it is more difficult to prepare larger crystals by biocrystallographic methods;

[0013] (8) The equipment is expensive

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