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Liquid microbe preparation for use in petroleum pollution degradation

A technology of microbial preparations and pollutants, applied in the field of bioremediation of oil-contaminated soil, can solve the problems of soil secondary pollution, accumulation of intermediate metabolites, etc., and achieve the effects of improved degradation ability, strong synergistic degradation ability, and good extensibility

Active Publication Date: 2006-07-12
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, fungi tend to produce toxic intermediate metabolites in the process of degrading organic pollutants, which may cause secondary pollution to the soil.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] In this example, the cultures of the fungus C. chinensis strain 1 and the bacterium Pseudomonas strain 1 were used to form a liquid microbial preparation.

[0014] The liquid microbial preparation used in this embodiment is prepared as follows:

[0015] A 300ml Erlenmeyer flask with a volume of 100ml liquid potato medium was inoculated with C. chinensis, cultured at 28°C and 160rpm for 3 days, and the culture was collected. The fungal content was 20mg dry weight / ml. Pseudomonas was inoculated in a 300ml Erlenmeyer flask with a volume of 100ml LB liquid medium, cultivated for 1 day at 30°C and 160rpm, and the culture was collected. The bacterial content was 10 8 pieces / ml. The obtained fungal culture and bacterial culture were mixed according to a mixing ratio of 10:1.

[0016] Collect farmland soil, and artificially prepare 2000g of petroleum-contaminated soil with a petroleum hydrocarbon content of 5%. After sterilization, the soil was divided into four small boxes ...

Embodiment 2

[0019] In an embodiment, a culture of the fungus Phanerochaete chrysosporium 1 strain and the bacterium Rhodococcus 1 strain was used to form a liquid microbial preparation.

[0020] The liquid microorganism preparation used in the embodiment is prepared as follows:

[0021] Phanerochaete chrysosporium was inoculated in a 300ml Erlenmeyer flask with a volume of 100ml liquid potato medium, cultured at 28°C and 160rpm for 3 days, and the culture was collected. The fungal content was 60mg dry weight / ml. Inoculate Rhodococcus in a 300ml Erlenmeyer flask with 100ml LB liquid medium, cultivate it for 2 days at 30°C and 200rpm, collect the culture, and the bacterial content is 10 12 pieces / ml. . The obtained fungal culture and bacterial culture were mixed according to a mixing ratio of 1:10.

[0022] Farmland soil was collected, and 2000 g of petroleum-contaminated soil with a petroleum hydrocarbon content of 3% was artificially prepared. After sterilization, the soil was divided...

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PUM

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Abstract

The invention relates to a liquid microorganism agent used in degrading petroleum pollution in the field of petroleum pollution soil biology mending technology. It separately dose fluid culture to the bacteria and fungus with petroleum hydrocarbon degradation capability and then mixes them to prepare for the liquid microorganism agent.

Description

technical field [0001] The invention relates to a liquid microbial preparation for degrading petroleum pollutants, which belongs to the bioremediation technology of petroleum-contaminated soil. Background technique [0002] Bioremediation technology is the main remediation technology for soil contaminated by petroleum and related products. Due to the complex composition, biodegradability and low bioavailability of petroleum pollutants, it is difficult for indigenous microorganisms to effectively, rapidly and completely degrade petroleum hydrocarbons in soil. The biological addition method can improve the efficiency of bioremediation and effectively remove petroleum hydrocarbon pollutants by adding microbial agents with high-efficiency degradation of petroleum hydrocarbons. Therefore, strengthening the breeding of dominant bacteria and developing environmental microbial preparations are the core of oil-contaminated soil bioremediation technology. There are a variety of micr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/20C09C1/10
Inventor 刘铮韩慧龙王福远佘鹏邢新会张敏莲苗长春江皓
Owner CHINA PETROLEUM & CHEM CORP
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