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Sea snail toxin variant polypeptide compound, its preparation process and application thereof

A peptide compound and conch technology, applied in the field of two peptide compounds, can solve the problems of high cost and insufficient raw material sources, and achieve the effect of fewer product purification steps

Inactive Publication Date: 2006-06-28
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Vc1.1 can be obtained directly from conch, but the source of raw materials is insufficient; it can also be obtained by chemical synthesis, but the cost is extremely high

Method used

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  • Sea snail toxin variant polypeptide compound, its preparation process and application thereof
  • Sea snail toxin variant polypeptide compound, its preparation process and application thereof
  • Sea snail toxin variant polypeptide compound, its preparation process and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0067] An alpha-conch toxin variant polypeptide compound, the amino acid sequence direction from the N-terminal to the C-terminal is:

[0068] AP2.1 GCCADPRDNYDHPEIC*

[0069] AP2.0 GCCADPRDNYDHPEIC

[0070] Among them, AP2.1 has amidated C-terminus, *—indicates amidation.

[0071] (1) Synthesize the gene encoding AP2.1 or AP2.0 and recombine it into the pET-32a(+) expression vector, and construct the genetic engineering bacteria for fermentation

[0072] 1. According to the codon preference of Escherichia coli, design the nucleotide sequence that can be inserted between the KpnI and EcoRI restriction sites of the pET-32a (+) vector in the correct reading frame: ggtacc gac gac gac gac aagGGTTGCTGCGCTGACCCGCGTTGCAACTACGACCACCCGGAAATCTGC taa gaattc (Order 5' to 3'). Among them, the underlined part is the restriction enzyme cleavage site, the italic part is the enterokinase cleavage site, and the uppercase part is the AP2.1 gene coding region, so that the AP2.1 gene coding ...

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Abstract

The invention discloses a manufacture method for two alpha-whelk toxin variant polypeptide compound, the gene coding the compound and the use of the compound. The polypeptide would be gained through the process of amino acid substitution, decorating, biology filtering, the amino acid sequence from N end to C end is: GCCADPRCNYDHPEIC. It is made by the method of chemical and gene engineering that has activity anti neuron nicotine acetylcholine receptor. It has high value in acceptor search and medicine developing.

Description

technical field [0001] The invention relates to two kinds of polypeptide compounds, their preparation method and their application as medicine, belonging to the field of biology. Background technique [0002] For a long time, drugs such as pethidine, methadone, and morphine have been widely used clinically to relieve intractable pain such as cancer pain, arthritis, and neuralgia. In the clinical application of this kind of drugs, there will be limitations such as increasing doses, tolerance, poor drug effect persistence and even addiction, which leads to many patients’ pain not receiving appropriate analgesic treatment, especially postoperative pain and Pain in morphine-insensitive patients. Therefore, screening new analgesic drugs with high efficacy, low tolerance, and long-lasting non-addiction has become necessary. [0003] Currently, one class of such drug candidates is represented by cysteine-rich polypeptides. They are chemically stable, have stronger analgesic effe...

Claims

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Application Information

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IPC IPC(8): C07K7/08A61P25/04C07K1/00C07K4/12C07K19/00C12N1/21C12N15/12C12N15/63C12P21/02
Inventor 张治国杨仑连桂芳徐朗莱
Owner NANJING AGRICULTURAL UNIVERSITY
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