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12 amino acid analog epi-position of human b cell specificity membrane molecule CD20 and polypeptide epi-position vaccine configurated by said analog epi-position

A technology of mimetic epitope and specificity, applied in the field of medicine and biology, can solve the problem of low homology, and achieve the effect of reducing the side effects of monoclonal antibodies and reducing the burden on patients

Inactive Publication Date: 2006-06-14
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

Sequence analysis showed that the conserved sequence of the phage peptide had very low homology with the amino acid sequence of the neu polypeptide chain, but each phage phenotype immunized animals could produce antibodies against HER2 / neu

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  • 12 amino acid analog epi-position of human b cell specificity membrane molecule CD20 and polypeptide epi-position vaccine configurated by said analog epi-position
  • 12 amino acid analog epi-position of human b cell specificity membrane molecule CD20 and polypeptide epi-position vaccine configurated by said analog epi-position
  • 12 amino acid analog epi-position of human b cell specificity membrane molecule CD20 and polypeptide epi-position vaccine configurated by said analog epi-position

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Embodiment Construction

[0076] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments.

[0077] According to the technical scheme of the present invention, to prepare the human CD20 molecular therapeutic autovaccine, the method of affinity screening is firstly applied, and Rituximab is used as a ligand to randomly present a 12-peptide library in phage (BioLabs  , New England) screened high-affinity 12 peptide mimotope Gln-Asp-Lys-Leu-Thr-Gln-Try-Pro-Lys-Try-Leu-Glu (glutamine-aspartic acid-lysine acid-leucine-threonine-glutamine-tryptophan-proline-lysine-tryptophan-leucine-glutamic acid), after chemically synthesizing the epitope, glutaraldehyde It is chemically coupled with KLH to obtain a vaccine for immunization.

[0078] To realize the screening of the peptide library of the present invention, the construction of the vaccine, and the immunization of animals, the work of antibody detection is completed according to the follow...

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Abstract

The present invention discloses a simulation epitope of 12 amino acids of human cell B specificity expression membrane molecule CD 20 and its polypeptide epitope vaccine constructed by using said simulation spitope. Said invention also provides a method for screening simulation epitope of CD 20 molecule by using Rituximab as ligand, and provides its amino acid sequence, Gln-Asp-Lys-Leu-Th-Gln-Try-Pro-Lys-Try-Leu-Glu. Said invention also provides a method for chemically-synthesizing simulation epitope of 12 amino acids and making it be chemically-coupled with keyhole limpet hemo cyanin (KLH) to obtain the successfully-constructed vaccine. Besides, said invention also provides the concrete application of said vaccine.

Description

technical field [0001] The invention belongs to the field of medical biotechnology, and specifically relates to screening of phage randomly presented peptide libraries, affinity detection and sequencing of screened phages, synthesis and coupling of polypeptides, animal immunization experiments, in vitro activity detection of induced antiserum and the like. It further relates to a 12-amino acid mimic epitope of human B cell-specific membrane molecule CD20 and a polypeptide epitope vaccine constructed by using the mimic epitope and capable of inducing autoantibodies against CD20 molecules in vivo. Background technique [0002] 1. Research on CD20 molecule and its monoclonal antibody [0003] 1.1 CD20 molecule [0004] The CD20 molecule is a marker molecule expressed on the surface of more than 95% of normal or deteriorated B cells, and is an ideal target antigen for the treatment of B cell lymphoma. The gene encoding CD20 is located in the t(11...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K39/00A61K39/395A61P35/00
Inventor 张英起李萌颜真
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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