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Spermatozoon acrosome enzyme activity quantitative determination reagent and detecting method thereof

A quantitative detection and acrosome enzyme technology, applied in biological testing, color/spectral characteristic measurement, measuring devices, etc., can solve the problems of low reaction efficiency, unfavorable clinical widespread development, complicated operation, etc., and achieve simplified operation procedures and reagent performance Stable and accurate response results

Inactive Publication Date: 2006-02-22
深圳华康生物医学工程有限公司
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Problems solved by technology

[0003] There are two types of acrosome enzyme assays: immunological assays and enzyme activity assays. Immunological assays are complex and reflect the total enzyme content rather than total enzyme activity; Sensitive human sperm arginine amidase activity, which should reflect all or almost all sperm acrosome activity, but due to its own methodology, the Kennedy method has low reaction efficiency and a detection time of up to three hours, which is not conducive to clinical practice Widely carried out

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  • Spermatozoon acrosome enzyme activity quantitative determination reagent and detecting method thereof

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[0026] Example, a reagent for quantitative detection of sperm acrosome enzyme activity, including (1) the inhibitor is a deionized aqueous solution containing sodium chloride, Hepes, Ficoll 400 and sodium azide, and each 100ml of the inhibitor contains 0.7 grams of sodium chloride , Hepes 0.3 g, Ficoll 400 is 5.5 g, sodium azide 0.05 g; (2) The buffer is a deionized aqueous solution containing sodium chloride, Hepes, BSA, sodium azide and Tween-2, and each 100ml buffer contains Sodium chloride 0.35 g, Hepes 0.6 g, BSA 0.5 g, sodium azide 0.05 g, Tween-2035 μl; (3) The stop solution is a deionized aqueous solution containing benzamidine, and every 100 ml of the stop solution contains benzene 8.73 g of formamidine; (4) The substrate solution is a dimethylformamide solution containing BAPNA, and each 100 ml of the substrate solution contains 0.75 g of BAPNA.

[0027] The manufacturing process method of the above-mentioned sperm acrosome enzyme activity detection reagent:

[0028...

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Abstract

This invention discloses one sperm acrosin activity quantifying testing agent, which comprises depressor, buffer liquid, terminal liquid and bottom liquid, wherein the said bottom liquid comprises N-ª

Description

technical field [0001] The invention relates to an in vitro diagnostic reagent, which is particularly suitable for quantitative detection of sperm acrosome enzyme activity. Background technique [0002] Sperm acrosome is a specialized lysosome containing a variety of hydrolytic enzymes, among which the acrosome enzyme activity can reflect the quality of sperm. Insufficient acrosome enzyme activity may lead to infertility, and it is difficult to identify the cause of infertility through semen routine. Otherwise, acrosome enzyme activity is often low. Therefore, acrosome enzyme activity is an important evaluation index for judging male sperm function and fertility, and can also be used as a basis for clinical assisted reproduction (ART) method selection. [0003] The acrosome enzyme assay can be divided into two categories: immunological assay and enzymatic activity assay. The immunological assay is complicated and reflects the total enzyme content, not the total enzymatic ac...

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Application Information

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IPC IPC(8): G01N33/50G01N33/573G01N21/31
Inventor 傅剑华刘瑜胡家纯何林何小红
Owner 深圳华康生物医学工程有限公司
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