Epidermal growth factor receptor (EGFR)gene sequencing detection method

Abstract

The invention relates to an epidermal growth factor receptor gene detection and analysis method, wherein the No. 18, 19 and 21 extron gene fragment of the EGFR can observe and analyze the gene sequence and mutation characteristic unique for the Chinese, on the level of bases and amino acids one by one. The No. 21 extron T>G mutation and A>T and No. 852 codon inserted G mutation discovered by this inventor are all novel heterozygous mutations not reported domestically and abroad, thus pertinency is achieved when used for identification of targeting medicinal treatment for the Chinese. The invention provides a process for detecting EGFR gene mutation, the result of which is used as the basis to decide whether to take medicines.

Description

technical field [0001] The invention relates to a gene detection method, more precisely, the invention relates to a detection and analysis method of epidermal growth factor receptor gene. It belongs to the technical field of molecular biology. Background technique [0002] Regarding the epidermal growth factor receptor (EGFR) and its small-molecule inhibitor gene-targeted drug gefitinib, the epidermal growth factor receptor family is a portal protein in the cell signaling system, which was first isolated in 1950. There are four known subtypes, Erb-B-1, Erb-B-2, Erb-B-3 and Erb-B-4. The most famous ones are Erb-B-1 and Erb-B- 2 subtypes, which are respectively determined by Her 1 and Her 2 Proto-oncogene production, Her 1 It is the functional gene that produces EGFR, Her 2 is to produce Neu(Cerb-B 2 ) functional gene. EGFR has three functional domains inside and outside the cell, namely the extracellular region, the transmembrane region and the cytoplasmic region. The ...

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Problems solved by technology

Problems and shortcomings of the existing technology: the epidermal growth factor receptor gene sequencing detection and analysis technology was first published in April 2004 by PaezJG and Lynch TJ of the United States in "sci e nce" and "New England Journal", they all used the full-length DNA gene of EGFR20 exons in the NCBI database, amplified the short fragment of EGFR106 genes by nested PCR method, and used the same gene sequencing method and instrument type get test result

At the end of 2004, the Japanese used total RNA extraction technology for CDNA PCR amplification, and applied this technology to the detection of molecular pathological genes in the clinical pathology department. Sexual single-strand gene mutation events increase false positive pollution or false negative missed detection, and increase the time and cost of detection, and lack clinical gene pathology application and report samples and technical operation specifications

[0015] 4. Domestic medical institutions and hospital departments have not yet set up the clinical genetic pathology detection project, and even failed to retrieve the EGFR exon detection reports or data from medical and biological laboratories

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