Method for preparing tobacco extractive by biological enzyme
A technology of tobacco extract and bio-enzyme, applied in the direction of preparation of tobacco, tobacco, application, etc., can solve problems such as hindering the development of the tobacco industry, affecting the aroma quality of cigarette products, and low nicotine content
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Embodiment 1
[0033] Take by weighing 100 grams of tobacco powder, pulverize them with a pulverizer, and the pulverizing particle size is 60-80 mesh. Then add them to the extraction kettle, add 600 grams of deionized water, and stir to make the powder evenly dispersed. Under the condition of stirring, rapidly heat and sterilize, the heating rate is 8°C / min, start to sterilize the water dispersion system of tobacco powder, raise the temperature to 85°C, and keep it for 5 minutes. After sterilization, cool the system quickly with water, and stop cooling when the temperature drops to 50°C. Under the condition of stirring, add 0.1% (W / W) cellulase, hemicellulase and pectinase respectively, the speed of stirring is controlled at 50 rpm, and the hydrochloric acid or sodium hydroxide of 0.1N is used to regulate the temperature of the system. The pH is 5.5, and the enzymatic hydrolysis time is 2 hours.
[0034] After the enzymolysis of cellulase, hemicellulase and pectinase is completed, 0.2% (W / ...
Embodiment 2
[0037] Take by weighing 100 grams of tobacco powder, pulverize them with a pulverizer, and the pulverizing particle size is 60-80 mesh. Then add them to the extraction kettle, add 800 grams of deionized water, and stir to make the powder evenly dispersed. Under the condition of stirring, rapidly heat and sterilize, the heating rate is 10°C / min, start to sterilize the water dispersion system of tobacco powder, raise the temperature to 80°C, and keep it for 10 minutes. After sterilization, cool the system quickly with water, and stop cooling when the temperature drops to 50°C. Under the condition of stirring, add 0.1% (W / W) cellulase, hemicellulase and pectinase respectively, the speed of stirring is controlled at 50 rpm, and the hydrochloric acid or sodium hydroxide of 0.1N is used to regulate the temperature of the system. The pH is 5.5, and the enzymatic hydrolysis time is 3 hours.
[0038] After the enzymolysis by cellulase, hemicellulase and pectinase was completed, 0.2% ...
Embodiment 3
[0041] Take by weighing 100 grams of tobacco powder, pulverize them with a pulverizer, and the pulverizing particle size is 60-80 mesh. Then add them to the extraction kettle, add 500 grams of deionized water, and stir to make the powder evenly dispersed. Under the condition of stirring, rapidly heat and sterilize, the heating rate is 10°C / min, start to sterilize the water dispersion system of tobacco powder, raise the temperature to 80°C, and keep it for 10 minutes. After sterilization, cool the system quickly with water, and stop cooling when the temperature drops to 50°C. Under the condition of stirring, add 0.3% (W / W) commercialized cellulase, hemicellulase and pectinase compound enzyme, the speed of stirring is controlled at 50 rev / min, with 0.1N hydrochloric acid or hydrogen The pH of the sodium-adjusted system was 5.5, and the enzymatic hydrolysis time was 2 hours.
[0042] After the enzymolysis of cellulase, hemicellulase and pectinase is completed, 0.2% (W / W) nicoti...
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