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Preparation method of vitelline antibody for resisting pstudorabies

A technology of porcine pseudorabies and egg yolk antibody, which is applied in the field of preparation of biological preparations, can solve problems such as troublesome procedures, high cost, and inconvenient feeding, and achieve the effects of high sensitivity, strong specificity, and simple method

Inactive Publication Date: 2006-01-25
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is inconvenient to raise pigs as experimental animals, the amount of blood collected at one time is small, and the procedures are cumbersome and costly
In a further literature search, no report identical or similar to the subject of the present invention "preparation method of anti-porcine pseudorabies egg yolk antibody" has been found

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Raise clean-grade laying hens, and immunize swine rabies vaccine for the first time, with an immunization dose of 0.5ml per head (the freeze-dried vaccine is diluted to 20ml with normal saline). The second immunization was carried out at an interval of 10 days, and the immunization dose was 0.5ml. After 10 days of immunization, the third immunization was carried out, and the immunization dose was 1 ml. Use enzyme-linked immunosorbent assay for titer determination. When the titer reaches 1:1280 or more, collect immunized eggs, aseptically obtain egg yolk, dilute with double-fold sterile water, and use 2mol.L -1 The pH of the lactic acid solution was adjusted to 4.4 for acid treatment to remove impurity proteins, 0.5% hydroxypropyl methylcellulose phthalate treatment and -40°C freeze-thaw treatment to remove lipids, and the suction filtrate was salted out with ammonium sulfate and dialyzed to remove High purity anti-swine rabies egg yolk antibody was obtained by salt and...

Embodiment 2

[0015] Raise clean-grade laying hens, and immunize pig rabies gE genetically engineered deletion vaccine for the first time, with an immunization dose of 0.5ml per head. The second immunization was carried out at an interval of 10 days, and the immunization dose was 0.5ml. After 10 days of immunization, the third immunization was carried out, and the immunization dose was 1 ml. The titer was determined by gE enzyme-linked immunosorbent assay, and the immunized eggs were collected when the titer reached 1:1280. The preparation method of anti-swine rabies egg yolk antibody is the same as scheme 1 thereafter.

[0016] The prepared egg yolk antibody has high sensitivity and strong specificity, which provides material conditions for the establishment of technologies and products of genetic engineering deletion vaccines and differential diagnosis methods; it becomes possible to use conventional serological methods to distinguish wild virus-infected pigs from vaccine-immunized pigs;...

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PUM

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Abstract

The invention discloses the process for preparing anti-Pseudorabies yolk (igY) which comprises, (1) employing pig's hydrophobia vaccine or pig's hydrophobia genetic engineering deletion vaccine purifying grade testing egg chickens, carrying out potency determination through enzyme linked immunoadsorption test, collecting immunological eggs, (2) reinforcing the immunological vitelline, carrying acid treatment through water dilution and lactic acid regulation, so as to remove impure proteins, carrying out phthalate methyl hydroxypropylcellulose treatment and freeze melting treatment to remove grease, desalinizing the filter liquor through ammonium sulfate salting out and dialysis, finally carrying out column chromatography to obtain the high purity anti-Pseudorabies igY with high sensitivity and strong specificity.

Description

technical field [0001] The invention relates to a preparation method of a biological agent, in particular, a preparation method of an anti-pseudorabies yolk antibody. used in the field of bioengineering. Background technique [0002] Pseudorabies is a disease caused by pseudorabies virus that can cause a variety of domestic and wild animals with fever, itching, and encephalomyelitis as the main symptoms. In recent years, with the large-scale and intensive development of my country's pig industry, pig pseudorabies has broken out in many areas, and there is a tendency to gradually expand and spread. The disease is seriously endangering the pig industry, and can cause abortion in pregnant sows, and most of the newborn piglets are acutely lethal, causing huge economic losses to the pig industry, and causing trade barriers in international trade. At present, the detection methods of the disease are mainly virus isolation, neutralization test, enzyme-linked immunosorbent assay (...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/08
Inventor 刘永德徐建雄叶陈梁汪毅
Owner SHANGHAI JIAO TONG UNIV
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