Scylla serrata antibacterial peptide and its genes and clone method for genes

Abstract

The present invention Scylla serrata antibiotic peptide and its gene and genetic cloning method, and relates to one kind of active antibiotic peptide scygonadin and its gene. Antibiotic peptide scygonadin is separated and purified from male sexual gland of Scylla serrata, and degenerate primer is designed and synthesized based on the total RNA of the male sexual gland as template and the detected 20 amino acid sequence in N end of antibiotic peptide scygonadin and cloned to the whole length cDNA gene sequence one the antibiotic peptide scygonadin by means of RT-PCR, RACE or other molecular biological process. Genetic engineering strain with high efficiency expression of the antibiotic peptide may be constituted via constituting eukaryotic expression vector of the gene so as to externally produce antibiotic peptide as feed additive for aquatic animal to resist drug resisting bacteria. In addition, the present invention may be used also in transgenic blue crab breeding.

Description

technical field [0001] The invention relates to an antibacterial active polypeptide scygonadin isolated and purified from the male gland of Scylla serrata (forskal), and the gene encoding the antibacterial peptide is cloned accordingly. Background technique [0002] Scylla serrata, English name mud crab, blue crab Latin name Scylla serrata (forskal), commonly known as blue crab, hairy crab, worm-seeking, scorpion, has a wide natural distribution and has its traces in temperate, subtropical and tropical sea areas. It is an important economic species for marine aquaculture. In recent years, with the breakthrough of artificial breeding technology of blue crab and the improvement of breeding technology, the breeding of blue crab has gradually changed from extensive farming to intensive farming. However, with the increase of breeding density, the phenomenon of eutrophication of aquaculture water has become more and more serious, resulting in the deterioration of the breeding env...

AI Technical Summary

Problems solved by technology

Therefore, antimicrobial peptides will be an antibacterial drug with broad application prospects in the prevention and treatment of marine crab diseases; however, natural antimicrobial peptides are extracted directly from crabs or artificially synthesized by chemical methods, which are limited by animal sources and chemical synthesis methods , it is difficult to obtain a sufficient amount of antimicrobial peptides, and the cost is expensive. Therefore, isolating and cloning antimicrobial peptide genes and using genetic engineering methods to produce large quantities of antimicrobial peptides in vitro is the key to obtaining high-yield antimicrobial peptides and ensuring their widespread application in aquaculture. premise

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