Hepcidin antibiotic peptide gene of weever cultured in sea water

Abstract

A hapcidin recombinant technology gene of blue pickerel, relates to the cloned hepcidin recombinant technology of blue pickerel.Using the liver RNA of blue pickerel as the model, consult the genetic sequence of cross blue pickerel hepcidin and human hepcidin to design the specific primer, makes use of RT-PCP, 5 race and 3race and so on, belongs to a new member in the hepcidin genetic family. With activity of antifungal, gram - positive bacteria and gram - negative bacteria, it is an iron adjusting hormone relative to iron metabolism, can be used to form expressive carrier of eukaryon for building genetic engineering metabolism for expressing hepcidin highly effective with feed immune additive, for producing hepcidin bacteria resistant peptide gene of blue pickerel in large quantity; to be feed immune additive, decreasing or eliminating the accumulation of antibiotics in aquatic products, increasing the quality of aquatic products; to be the effective replacement of antibiotics with chemicals proof for medicine of germ with chemicals proof in cultivating fishery and breeding research of transgenetic fish.

Description

technical field [0001] The invention relates to a hepcidin antimicrobial peptide gene cloned from sea bass (Lateolabrax japonicus). Background technique [0002] Mariculture perch (English name Japanese seabass, Latin name Lateolabrax Japonica, commonly known as flower perch, seven-star perch, perch board) is an important sea-farming economic fish. In recent years, the culture status of perch has been rising continuously in our country, and the cultivation of perch in net cages and ponds has been carried out along the coast of the country. However, with the increase of breeding density, the phenomenon of eutrophication in the breeding water body is becoming more and more serious, which leads to the deterioration of the breeding environment, and the continuous outbreak of viral diseases, bacterial diseases, fungal diseases and parasitic diseases, which not only cause huge economic losses, but also seriously contus increased people's enthusiasm for farming. [0003] Antibact...

AI Technical Summary

Problems solved by technology

Therefore, antimicrobial peptides will be a kind of antibacterial drugs with broad application prospects in the prevention and treatment of marine fish diseases; however, natural antimicrobial peptides are extracted directly from fish or artificially synthesized by chemical methods, which are limited by animal sources and chemical synthesis methods , it is difficult to obtain a sufficient amount of antimicrobial peptides, and the cost is expensive. Therefore, isolating and cloning antimicrobial peptide genes and using genetic engineering methods to produce large quantities of antimicrobial peptides in vitro is the best way to obtain high-yield antimicrobial peptides and ensure their large-scale application in aquaculture. premise

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