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Method for early detecting gastric cancer from blood serum

An early detection, serum technology, applied in the field of early detection of malignant tumors, can solve the problems of electrophoretic separation and detection of insoluble proteins

Inactive Publication Date: 2005-12-14
SECOND AFFILIATED HOSPITAL ZHEJIANG UNIV COLLEGE OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the past, proteomics research mainly relied on two-dimensional electrophoresis (Two Dimensional Electrophoresis, 2DE), and the number of detected proteins was less than the estimated total protein in the cell. However, the general 2DE can distinguish 1000-3000 protein spots, which is not suitable for low-copy proteins in cells. However, the electrophoretic separation and detection of extremely acidic or basic proteins, proteins with too large or too small molecular weight, and insoluble proteins (including membrane proteins) are still facing great difficulties

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  • Method for early detecting gastric cancer from blood serum
  • Method for early detecting gastric cancer from blood serum
  • Method for early detecting gastric cancer from blood serum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Serum sample preparation:

[0029] (1) All serum samples were thawed in an ice bath for 30-60 minutes and then centrifuged for 5 minutes. Take 10ul of serum and 20ul of denaturation buffer U9 (9M Urea, 2% CHARPS, 50mM Tris-HCl PH9) and mix thoroughly. Medium shaking for 30 minutes;

[0030] (2) Carefully take out the SAX chip and mark it, put it into the biochip processor, add 200ul SAX binding buffer to each well and shake twice to pretreat the chip;

[0031] (3) Take 10ul of the sample and 110ul of SAX binding buffer (50mM Tris-HCl pH9) solution and mix quickly to avoid bubbles, add 100ul of the processed serum sample to each well, and shake at 4°C for 60 minutes;

[0032] (4) After serum samples, add 200ul SAX binding buffer to each well, shake twice at room temperature, then add 200ul deionized water to wash the chip twice;

[0033] (5) Take out the chip, add 0.5ul SPA to each well after micro-drying, and repeat once after micro-drying;

[0034] (6) On 5 machi...

Embodiment 2

[0049] 1. Serum sample information:

[0050] The 86 serum samples were all collected from the Second Affiliated Hospital of Zhejiang University School of Medicine from February 2003 to November 2003 with the informed consent of the hospital ethics committee and the patients. Non-gastric cancer healthy volunteers with gastroscopic examination and pathologically confirmed chronic superficial gastritis or chronic atrophic gastritis without a history of malignant tumors. Among the patients, there were 31 males and 14 females, with an average age of 56.5 years (36-76 years old). UICC tumor stages: 14 cases of stage I, 6 cases of stage II, 10 cases of stage III, and 15 cases of stage IV. The control group was paired by age and sex, including 28 males and 13 females, with an average age of 52.6 years (38-73 years). All serum samples were drawn before treatment on an empty stomach in the morning, and the serum was stored in a -80°C low-temperature refrigerator.

[0051] 2. Instrumen...

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Abstract

The early stage stomach cancer detecting method with serum is to utilize fingerprint comprising ten proteins of charge / mass ratio 4141 Da, 4474 Da, 3940 Da, 4633 Da, 4792 Da, 3957 Da, 4976 Da, 5348 Da, 4096 Da and 5915 Da separately in detection. The detecting method includes the following specific steps: preparing serum, collecting mass spectrum data, and biological informatics analysis of the collected data. The present invention provides new concept and method for the early stage diagnosis of stomach cancer and lays foundation of new tumor marker. The method of the present invention is reasonable and feasible, and has combined biological informatics method and analysis method with specificity and sensitivity over 84 % each and greatly raised stomach cancer detecting rate.

Description

technical field [0001] The invention relates to a method for early detection of malignant tumors, which is a new non-invasive detection method for early detection and early detection of gastric cancer, and its sensitivity and specificity both reach more than 84%. Background technique [0002] Gastric cancer is the second most common malignancy worldwide and remains an important cause of cancer death. In my country, the incidence rate of gastric cancer is about 20 / 100,000, and the death rate ranks second in the cancer mortality rate in my country. Most of the patients are in the middle and late stage, and the prognosis is poor. According to statistics, the 5-year survival rates of radical surgery for early gastric cancer and advanced gastric cancer are 90% and 40%, respectively. Therefore, only early detection and early diagnosis can benefit more gastric cancer patients, but early gastric cancer is mostly asymptomatic or has non-specific symptoms. Heterosexual symptoms. At pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 黄建邱福铭陈益定郑树
Owner SECOND AFFILIATED HOSPITAL ZHEJIANG UNIV COLLEGE OF MEDICINE
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