Methods and compositions for accelerating alcohol metabolism

A technology of ethanol metabolism and composition, which is applied in the field of composition to accelerate ethanol metabolism, and can solve problems such as limited effects

Inactive Publication Date: 2005-12-14
ENZYMEDIX
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Nevertheless, all existing methods and compositions for reducing the health hazards of alcohol consumption and hangovers have only limited effectiveness

Method used

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  • Methods and compositions for accelerating alcohol metabolism
  • Methods and compositions for accelerating alcohol metabolism
  • Methods and compositions for accelerating alcohol metabolism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1 description.

[0068] B. Coupling regeneration of NAD

[0069] As shown in Formula 1 and Formula 2 above, the mechanism of ethanol metabolism catalyzed by ADH and ALDH enzymes requires the coenzyme NAD. Oxidation of each mole of ethanol consumes 2 moles of NAD. Since NAD is consumed, the redox ratio, that is, NAD + / NADH ratio, is reduced so that the oxidation of ethanol is limited by the limited supply of NAD. NAD thus becomes the limiting factor for ethanol metabolism.

[0070] Therefore, to maintain an efficient rate of ethanol oxidation catalyzed by ADH, regeneration of NAD from NADH is required + , and this NADH is produced by the reaction of ADH as shown in formula 1 and formula 2, so that the redox ratio, NAD + / NADH, remained relatively unchanged.

[0071] In humans and other mammals, the redox ratio NAD + / NADH is regulated by a number of enzymes, including lactate dehydrogenase (lactate dehydrogenase), β-hydroxybutyrate dehy...

Embodiment

[0093] Embodiment 1. Preparation of animal gland extract

[0094] Fresh animal gland parts are washed and perfused with a liquid such as ice-cold saline, then homogenized in a kitchen homogenizer with, for example, 4 volumes of ice-cold buffer solution (0.1M potassium phosphate, pH=7.8) and 1 mM sodium bisulfite . All the following manipulations were performed at 0-4°C using the same buffer containing 0.1M sodium bisulfite. The homogenate was then centrifuged at about 20,000 g for about 30 minutes. The supernatant (extract I) was fractionated by addition of ammonium sulfate (30-50% saturation), resulting in a precipitate. The precipitate (extract II) was isolated by centrifugation at 5000g for 10 minutes. The pellet was redissolved in a small volume of buffer. Cold acetone (-10°C) was added to the solution, resulting in a precipitate (Extract III). The solid is further purified by ion exchange chromatography, gel filtration, and / or affinity chromatography (Extract IV), if...

Embodiment 2

[0097] Example 2. Enzyme activity of pig liver extract

[0098] The animal gland extract prepared in Example 1 was tested for various enzyme activities: alcohol dehydrogenase, acetaldehyde dehydrogenase, lactate dehydrogenase, sorbitol dehydrogenase and diaphorase, as follows.

[0099] a. Alcohol dehydrogenase

[0100] The activity of alcohol dehydrogenase (catalyses the conversion of ethanol to acetaldehyde, Formula 1) was determined spectrophotometrically. As shown in Formula 1, the reaction at pH=9 (such as Tris or phosphate buffer) is favorable for the formation of acetaldehyde, and the formed acetaldehyde is coupled with a capture agent. NADH has a maximum absorption at 340nm. A unit of enzyme activity is defined as an increase in absorbance per minute at 35°C (1 unit). In each test, 3.0 mL " ADH Cocktail Solution". Changes in absorbance at 340 nm were followed after addition of 10 or 20 μL of animal gland extract. Reported activity is the average of at least 6 dete...

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Abstract

The present invention provides compositions for accelerating ethanol metabolism and methods of use thereof, the compositions being selected from the group consisting of NAD, NADH oxidation co-substrates, precursors thereof, and combinations thereof, to promote regeneration of NAD that catalyzes ethanol metabolism, Drunkenness is thereby reduced and hangovers prevented by administering to the user a composition containing an amount of the extract effective to reduce ethanol intoxication.

Description

technical field [0001] The present invention generally relates to compositions for accelerating the metabolism of ethanol. Background technique [0002] Ethanol has been used worldwide throughout history. Moderate consumption of alcoholic beverages is acceptable socially. Many people believe that ethanol adds flavor to food. In addition, moderate consumption of alcoholic beverages is believed to have health benefits due to reduced stress and fewer heart attacks. It has also been reported that, in addition to fewer heart attacks and strokes, moderate consumers of alcoholic beverages (beer, wine, or liquor) are generally less likely to suffer from high blood pressure, general arterial disease, Alzheimer's disease, and colds. [0003] However, drinking large amounts of ethanol can have serious consequences. When alcoholic beverages are consumed, the ethanol passes into the stomach and soon reaches the small intestine. Ethanol enters the bloodstream from the small intestine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/55A61K36/064
CPCA23V2002/00A61K35/55A61K36/064A61K2300/00A23V2200/334
Inventor 王详槐
Owner ENZYMEDIX
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