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Application of liver cell growth factor in preventing and treating tissue accretion

A technology of hepatocyte growth factor and tissue adhesion, applied in gene therapy, medical preparations containing active ingredients, drug combinations, etc., can solve problems such as side effects and insignificant curative effect, and achieve a clear effect

Active Publication Date: 2005-11-23
RADIOLOGY INST ACAD OF MILITARY MEDICINE SCI PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, research on the prevention and treatment of postoperative tissue adhesions has found that antibiotics, antihistamines, corticosteroids and other anti-inflammatory drugs, dextran and tissue plasminogen activator have a certain role in the prevention and treatment of postoperative tissue adhesions; It is found that some local barrier agents that can be decomposed and absorbed by tissues, such as chitosan, also have certain effects, but the curative effect is not significant and there are certain side effects

Method used

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  • Application of liver cell growth factor in preventing and treating tissue accretion

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Embodiment Construction

[0019] 1. Recombinant plasmid pcDNA3H carrying human hepatocyte growth factor gene and pUDKH or recombinant adenovirus Ad-HGF

[0020] (1) Construction of pcDNA3H plasmid

[0021] Using conventional molecular biology techniques, from the human placenta cDNA library (purchased from Clontech Company, the United States) by polymerase chain reaction (PCR) (upstream primer is 5'-ccatcgatgttaacatgtgggtgaccaaactc-3', downstream primer is 5'-tgggatccgccgccgcctatgactgtggtacctt-3' ) to isolate the human HGF coding region cDNA (2184bp), and then subclone it into the multiple cloning site (BamH I, Apa I site) of the eukaryotic expression vector pcDNA3 (purchased from Invitrogen Company, the U.S.), to obtain human liver The recombinant vector pcDNA3H of the cell growth factor gene, the human hepatocyte growth factor gene is regulated by the CMV promoter. The EGFP (green fluorescent protein) gene sequence was excised from the vector pEGFP-N1 (purchased from Invitrogen, the U.S.) with restr...

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Abstract

The invention relates to biology and medicine fields, concretely, relates to application of liver cell growth factor in preventing and treating tissue accretion. The invention inserts human hepatocyte growth factor full-length coding region cDNA into plasmid carrier (pc DNA 3 or p UDK or others), or into adenovirus carrier (such as Ad5 or gutless adenovirus) to obtain recombinant plasmid or recombinant adenovirus of the human hepatocyte growth factor gene. The recombinant plasmid or recombinant adenovirus can prevent and cure postoperative intestinal adhesion or pelvic cavity adhesion or other tissue adhesion by being locally painted on abdominal cavity or pelvic cavity. Thus, the recombinant plasmid or recombinant adenovirus carrying the human hepatocyte growth factor gene has excellent application prospect on prevent-curing or treatment for postoperative tissue adhesion.

Description

field of invention [0001] The invention relates to the application of hepatocyte growth factor in preventing or treating tissue adhesion. technical background [0002] Intestinal adhesions after surgery or pelvic adhesions after pelvic surgery are still one of the intractable difficulties clinically. 70-80% of the adhesions are caused by surgical operations. Postoperative intestinal adhesions or pelvic adhesions can cause intestinal obstruction, female infertility, and local repeated long-term pain. Severe cases have to undergo secondary operations. At present, research on the prevention and treatment of postoperative tissue adhesions has found that antibiotics, antihistamines, corticosteroids and other anti-inflammatory drugs, dextran and tissue plasminogen activator have a certain role in the prevention and treatment of postoperative tissue adhesions; It is found that some local barrier agents that can be decomposed and absorbed by tissues, such as chitosan, also have cer...

Claims

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Application Information

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IPC IPC(8): A61K38/18
Inventor 刘红军吴祖泽哈小琴吴彬毕建进唐仲雄王立生鲁茁壮段海峰
Owner RADIOLOGY INST ACAD OF MILITARY MEDICINE SCI PLA
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