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Multifunctional photoexcited surface plasma resonant imager

A surface plasmon and resonance imaging technology, applied in the field of detection, can solve the problems of inability to realize the correlation between SPR spectrum and imaging or function conversion, complex production process, small scalability, etc., and achieve simple structure, flexible instrument structure, and production The effect of the scheme is simple

Inactive Publication Date: 2005-06-22
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In order to improve the measurement capacity and the measurement speed per unit time, the United States has developed a cross-microchannel SPR imaging system for measuring the interaction of DNA and RNA and applied for a related patent (PUB.APP.NO.20030017579), which uses silicon rubber to make a cross Microchannel, but the production process is complicated and the practicability is poor
There is also a latest patent in the United States (PUB.APP.NO.20030048452) about the system of SPR two-dimensional imaging, but this patent is still a single-function instrument, that is, it only involves two-dimensional imaging, the scalability is small, and the SPR spectrum cannot be realized Association or functional transformation with imaging

Method used

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  • Multifunctional photoexcited surface plasma resonant imager
  • Multifunctional photoexcited surface plasma resonant imager
  • Multifunctional photoexcited surface plasma resonant imager

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] The bottom of the prism is coated with a 50nm-thick gold film as a highly reflective sensing film. The sensing film is first treated with mercaptoundecanoic acid, and bovine serum albumin dots are assembled by chemical modification. The instrument uses CCD as the detector, and the surface plasmon resonance image in the solution is shown in Figure 6 , Figure 7 , Figure 8 Three-dimensional plots and linear overlay plots, respectively.

Embodiment 2

[0069] The same method was used to modify the sensing membrane as in Example 1, respectively modifying bovine serum albumin and polylysine. The instrument uses CCD as the detector, and the surface plasmon resonance image in the solution is shown in Figure 9 (The third column on the left is bovine serum albumin, and the third column on the right is polylysine), Figure 10 for its three-dimensional image.

Embodiment 3

[0071] A CCD was used as a detector and a grating was used to fix the angle of incident light, and a similar method was used to modify the sensing film as in Example 1, and the sensing film was connected with bovine serum albumin. First pass the buffer solution into the sample cell, and then pass the glucose sample prepared in the same buffer solution, and start the real-time detection operation at the same time, record the change of the resonance wavelength of the buffer solution and the glucose solution respectively, see Figure 11 , thereby realizing real-time and dynamic analysis of glucose.

[0072] SPR software process of the present invention, see Figure 12 to Figure 17 .

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Abstract

This invention multifunction imager is made based on the surface plasma resonance phenomenon composed of p-polarized parallel light sources, a light incident angle modulation system, a sample transport and analysis system, a reflection light cut-away and modulation system, a detector, and a data register and process system. Said device utilizes the dispersion of P-polarized light and a prism to activate the resonance of sensing film surface plasma to test resonance signals. The resonance image signal or wave length scan resonance signal can be tested by conversion of light sources, the chip research result can be reached by micro-lattice process to sense film to realize spectrum conversion of image and resonance wavelength.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a light-excited surface plasmon resonance instrument system. Specifically, it converts the light-excited surface plasmon resonance signal into an intuitive image, and can also be converted into a resonance wavelength detection system. Background technique [0002] Surface Plasmon Resonance (SPR) is based on the resonance caused by the excitation of surface plasmons by P-polarized light. When P-polarized light propagates in a high-refractive medium such as glass and projects on a thin layer of free electrons such as silver or gold film at its interface, resonance can be formed at a certain angle or wavelength, resulting in reflection attenuation, that is, at a certain angle (resonance Angle) or wavelength (resonance wavelength) measured reflected light intensity minimum, forming an inverted peak. The resonance angle or resonance wavelength is very sensitive to the change of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/63
Inventor 陈义黄昊文余晓齐莉
Owner INST OF CHEM CHINESE ACAD OF SCI
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