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Method for screening aerobic denitrifying bacteria

An aerobic denitrifying bacteria, oxidation state technology, applied in biochemical equipment and methods, bacteria, microorganism measurement/inspection, etc., can solve problems such as high engineering cost, secondary pollution, and poor system impact resistance.

Inactive Publication Date: 2005-03-16
HARBIN INST OF TECH
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  • Claims
  • Application Information

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Problems solved by technology

However, the traditional biological denitrification technology, because the demand for oxygen of nitrifying bacteria and denitrifying bacteria is completely opposite, it is difficult to unify the two processes of nitrification and denitrification in time and space, and has the following disadvantages: (1) High engineering cost, The operation control is complicated; (2) The acidity generated in the nitrification process needs to be neutralized by adding alkali, which not only increases the treatment cost, but also may cause secondary pollution; (3) The system has poor impact resistance and high concentration of NH 4 + , NO 2 - inhibits the growth of nitrifying bacteria

Method used

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  • Method for screening aerobic denitrifying bacteria
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specific Embodiment approach 1

[0005] Specific embodiment 1: This embodiment is carried out according to the following steps: a. Enrichment of aerobic denitrifying bacteria: adopt the method of domesticating anaerobic denitrification sludge to enrich the biological flora, the interaction between microorganisms and the environment, microorganisms and microorganisms The effect is complex and subtle. Sudden changes in environmental conditions (from anaerobic / anoxic to aerobic) will not only fail to enrich the effect, but will also destroy the original ecological balance and damage the original flora, so adopt Means of acclimatizing anaerobic denitrification sludge (acclimatizing devices such as figure 1 As shown), gradually change the environmental conditions, that is, gradually increase the aeration rate, aeration time, and the concentration of nitrate and nitrite in the influent, to achieve the environmental conditions for the strains to be screened, and give the biological flora a process of adaptation, and ...

specific Embodiment approach 2

[0006] Specific implementation mode two: this implementation mode is realized in this way:

[0007] a. Enrichment of aerobic denitrifying bacteria: (1) Take activated sludge from SBR method for treating high-concentration ammonia-nitrogen wastewater or traditional anaerobic denitrification sludge process; (2) Initial domestication, only use FM distribution water as influent , every 2 to 3 days, increase the content of the DM water distribution culture solution in the distribution water until all the distribution water is DM culture solution, the COD of the influent water is controlled at 5000 mg / L, the total nitrogen (TN) is at 500 mg / L, and during the acclimation process In the process, after maintaining the original dissolved oxygen (DO) concentration of the sludge for 2 to 3 days, while changing the water distribution, DO is also increased by 0.5 to 1 mg / L. The second is to gradually reach the suitable conditions for aerobic denitrifying bacteria, so that the bacteria can b...

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Abstract

The invention relates to a high efficiency screening method for aerobic denitrifying bacteria. The invention includes the following steps: a. enriching bacteria employing domesticating anaerobic denitrifying sludge, b. separating and purifying domesticated bacteria from sludge using plate dilution method and plate streak method, c. preliminary screening for pure bacteria after separation and purification, obtaining bacteria with 50% TN removal rate, d. screening bacteria again, obtaining aerobic denitrifying bacteria. Accomplishment of aerobic denitrifying technology can not only overcome the shortcomings of traditional biological denitrification technology, but possess the following advantages: (1) can accomplishing denitrification, COD removal in same reactor, (2) shortening retention time under feedback regulation, (3) diminishing aeration volume, (4)alkaline denitrification produced can neutralizing with acid produced in nitration helping to realizing short range synchronous nitration and denitrification.

Description

Technical field: [0001] The invention relates to a method for efficiently screening aerobic denitrifying bacteria. Background technique: [0002] It is generally believed that denitrifying bacteria that perform nitrate respiration are facultative anaerobic microorganisms, because only under anaerobic conditions can they induce nitrate reductase A and nitrite reductase required for denitrification. However, in recent years, people have continuously found the loss of total nitrogen under aerobic conditions, so the research on aerobic denitrification has gradually attracted the attention of researchers. At present, the cause of aerobic denitrification phenomenon is still inconclusive. Most researchers at home and abroad study aerobic denitrification technology from the production of local anaerobic or micro-environment anaerobic. The operation control is complicated and the system is unstable. Nitrogen in water can cause eutrophication, so the removal of nitrogen has attracted...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/02
Inventor 马放周丹丹任南琪杨基先
Owner HARBIN INST OF TECH
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