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Application of recombinant alpha-interferon in preparing medicine for preventing and treating respiratory system infection

A technology of recombinant human interferon and alpha interferon, applied in the field of biopharmaceuticals

Inactive Publication Date: 2004-11-24
BEIJING TRI PRIME GENE PHARMA CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although alpha interferon has been used in the treatment of many diseases, the main indication is chronic disease caused by virus, and there is no literature report that it can be used for acute disease caused by virus, such as SARS (severe acute respiratory syndrome), etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0010] In HELA cell culture, the inhibitory effect of different concentrations of recombinant interferon on coronavirus and rhinovirus was observed by cytopathic inhibition (CPE) method.

[0011] 1. Materials

[0012] 1) Drugs: recombinant human interferon α1b, recombinant integrated interferon α and mutants (multiple molecules in the application number 02159950.5), interferon α2a and α2b.

[0013] 2) Positive control drug: Ganciclovir for injection.

[0014] 3) Cells: human cervical carcinoma cells (HELA).

[0015] 4) Viruses: Coronavirus No. 9 (isolated from throat swabs of SARS patients) and rhinovirus (isolated from throat swabs of patients with common cold).

[0016] 2. Experimental method

[0017] 1) Toxicity determination method of interferon to HELA cells

[0018] HELA cells were inoculated into 96-well culture plates at a concentration of 400,000 / ml at 37°C, 5% CO 2 After culturing for 24 hours, add interferon, interferon is diluted to 25mg / ml-1.0ug / ml, and ganci...

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Abstract

The alpha-interferon has been used mainly in treating chronic viral infection. The present invention uses coronavirus or rhinovirus infected human cervical cancer cell as estimation model in observing the inhibition effect of alpha-interferon on common virus causing respiratory system infection. The results show that alpha-interferon may be used in preventing and treating coronavirus and rhinovirus caused diseases, including SARS. The integrated interferon, its mutant and interferon alpha-1b have even high inhibiting effect on new SARS virus.

Description

1. Technical field [0001] The invention belongs to the field of biopharmaceuticals, and mainly relates to the application of recombinant α Interferon (recombinant α Interferon) in the preparation and prevention of diseases caused by coronaviruses, rhinoviruses, etc. 2. Background technology [0002] Interferon has a wide range of anti-virus, anti-tumor and immune regulation functions. Its mechanism of action is to effectively curb virus invasion by inducing a variety of anti-viral proteins, inhibiting virus replication in cells, enhancing NK cell activity and other immune regulation functions and the occurrence of infection, and has various functions such as inhibiting the growth of tumor cells and clearing early diseased cells. Because interferon has the above-mentioned important functions, people's research on it is also deepening, and new interferon molecules are constantly being discovered. Currently, there are mainly 9 known interferon molecules: interferon-α: genus I ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/21A61P1/14A61P31/16C07K14/56
Inventor 刘金毅刘永全孙俭波程永庆
Owner BEIJING TRI PRIME GENE PHARMA CO LTD
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