Process of continuous production of casein bioactive peptide by enzymolysis and filtering membrane concentration

Abstract

The invention relates to a process of continuous production of casein bioactive peptide by enzymolysis and filtering membrane concentration which comprises, using caseinum as raw material and at least one prolease action, obtaining biologically active polypeptides having multiple functions in multi-stage enzyme membrane reactor combined from enzymolysis tank and hyperfiltration, nano filter membrane of dissimilar entrapment molecular weight.

Description

technical field [0001] The invention relates to a new process for continuously producing casein-derived polypeptides by integrating enzymatic hydrolysis reaction and membrane separation technology, and belongs to the technology for producing active polypeptides by enzymatically hydrolyzing casein as a substrate. Background technique [0002] Casein is the protein with the highest content in milk. It is a complex of several phosphorus-containing proteins, and its main component is α s1 、α s2 , β, κ four polypeptide chains. Casein is hydrolyzed by a single enzyme or a complex enzyme system, and then separated and purified to obtain nearly 10 categories of more than 100 biologically active polypeptides. Complex enzymatic hydrolysis products and diverse sequence structures make this kind of substances have various physiological functions such as immune regulation, free radical scavenging, mineral carrier, anticancer and antibacterial, thrombolysis and blood pressure reduction,...

AI Technical Summary

Problems solved by technology

[0004] At present, intermittent enzymatic hydrolysis is widely used in the production process of casein bioactive polypeptides, that is, batch operations are carried out in ordinary hydrolysis tanks. Casein is precipitated and centrifuged, followed by multi-step separation and purification steps such as solvent selective precipitation or ion exchange resin treatment to obtain the target polypeptide product. The following problems exist in the process: (1) The enzymatic hydrolysis product cannot be obtained from the reaction Timely separation in the system is likely to cause product inhibition, and the conversion rate of the enzymatic hydrolysis reaction and the yield of the target polypeptide are both low; (2) There are certain differences in the hydrolysis conditions between different reaction batches, and the product quality is unstable; (3) From the reaction It is very difficult to separate the product in the system and recover the enzyme from the reaction mixture, so the enzyme consumption is large, the process cost is high, and it cannot fully meet the requirements of commercialization

Images