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Extracting and separating method of novel langdu root essence B from stellera chamaejasme L and its uses

A new technology of wolf toxin and Ruixiang wolf toxin, applied in the application, botanical equipment and method, effective components of heterocyclic compounds, etc., can solve the problems of low extraction efficiency, complicated separation method, unsuitable for industrial scale production, etc. Simple separation method and high extraction efficiency

Inactive Publication Date: 2004-11-10
NORTHWEST A & F UNIV
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Problems solved by technology

These studies have a common feature, that is, they all belong to the basic theoretical research of phytochemistry or natural product chemistry. The research methods are all conventional methods in the laboratory, the extraction efficiency is low, the separation method is complicated and cumbersome, and it is not suitable for industrial scale production at all.
Secondly, all the research reports on nemethastoxin B seldom involve the research on its biological activity, and there is no research on the effect of nemethoresin B on plant pathogenic bacteria, that is, as a plant fungicide

Method used

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  • Extracting and separating method of novel langdu root essence B from stellera chamaejasme L and its uses
  • Extracting and separating method of novel langdu root essence B from stellera chamaejasme L and its uses
  • Extracting and separating method of novel langdu root essence B from stellera chamaejasme L and its uses

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Embodiment Construction

[0028] 1. See figure 1 , the present invention adopts 4-step method to obtain different contents of neomayerina toxin B, that is, extraction, crude fractionation by silica gel column chromatography for the first time, crude fractionation by silica gel column chromatography for the second time, purification by sephadexLH-20, and separation obtained by each step The contents of neochamaerin B in the target substance are 5%-25%, 45%-55%, 80%-90%, and 95%-99%, respectively. Its technological process is as follows:

[0029] 1.1. Crush the root of chamaejasma to a certain extent, and its fineness is not strictly required, generally 1mm in diameter. According to the ratio of the weight (kg) of the wolfba root powder and the solvent volume (L) of 1:8, intermittent ultrasonic extraction is adopted, and the extraction temperature is 40°C. Each batch of raw materials was continuously extracted three times with the same solvent, and the extraction time was 40 min each time. The ultraso...

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Abstract

The invention relates to a process for extracting and separating novel radix euphorbiae ebracteolatae B from Daphnetin radix euphorbiae ebracteolatae and use thereof, comprising the steps of, using Daphnetin radix euphorbiae ebracteolatae as raw material, using 4-10 times weight of ethyl acetate or acetone or mixture of ethyl acetate and acetone as solvent, extracting three times continuously by adopting 30-55 deg. C ultrasonic wave under normal pressure, the time for each extraction being 30-50 mins, wherein 60%-90% novel radix euphorbiae ebracteolatae B can be prepared through filtering, concentrating and column chromatography chromatography.

Description

1. Technical field: [0001] The present invention relates to a comprehensive utilization of Daphne chamaejasmin in the field of medicine, veterinary medicine and pesticide intermediates or original medicines, in particular to a method for extracting and separating Neochachamasin B from Daphne chamaejasme and its application as a plant fungicide. 2. Background technology: [0002] Stellera chamaejasme (Stellera Chamaejasme L.) is a plant of the genus Stellera chamaejasme, and its aliases include chamaejasme, chrysanthemum, etc. Its root is the poisonous matrix of traditional Chinese medicine, which has the functions of dispelling stagnation, dispelling water, relieving pain and killing insects. As early as 1959, in the "China Soil Pesticides", there is a record of the application of chamaejasma chamaejasme as a botanical pesticide. The water extract is sprayed on the leaves of plants to prevent and control leaf bean aphids, cabbage aphids, leaf rust, etc. In recent years, som...

Claims

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Application Information

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IPC IPC(8): A01N43/16A61K31/35C07D407/04
Inventor 周乐
Owner NORTHWEST A & F UNIV
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