Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Identifying and comparing method for plant medicinal material

A technology of medicinal materials and plants, which is applied in the field of identification and comparison of specific varieties of medicinal materials based on chloroplast DNA sequences, which can solve the problems of misuse, mixed use and fraudulent use of medicinal materials

Inactive Publication Date: 2004-09-29
IND TECH RES INST
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the market of traditional Chinese medicinal materials has been investigated by scholars and experts, and it is found that various medicinal materials are misused, mixed and used fraudulently.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Identifying and comparing method for plant medicinal material
  • Identifying and comparing method for plant medicinal material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0058] The nonsense meson used in the method of the present invention can be any nonsense meson in the chloroplast DNA map, and it is preferably a nonsense meson located between the psbA gene and the trnH gene when applied to Scutellaria plants; the method of the present invention is applicable The plant medicinal materials are not limited; in the method of the present invention, the following primer pairs are preferred:

[0059] 5’-TGATCCACTTGGCTACATCCGCC-3’

[0060] 5’-GCTAACCTTGGTATGGAAGT-3’

[0061] The nonsense meson between the psbA gene and the trnH gene in the chloroplast DNA fragment extracted from Scutellaria baicalensis Georgi was subjected to a polymerase chain reaction.

[0062] In order to enable the examiner to better understand the technical content of the present invention, a preferred specific embodiment is described as follows.

[0063] The scutellaria medicinal materials used in this example were collected from various places.

[0064] (1) The medicinal materia...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The plant medicinal material identifying and comparing method includes mainly the following steps: providing the first chloroplast DNA sequence of the first plant medicinal material sample; provides the second plant medicinal material sample; extracting the total DNA from the sample; PCR proliferating the extracted plant medicinal chloroplast DNA segment containing one nonsense meson; sorting the chloroplast DNA segment to obtain the chloroplast DNA sequence of the second sample; and comparing the first chloroplast DNA sequence and the second chloroplast DNA sequence to determine whether the second sample plant medicinal material is the same as the first one.

Description

Technical field [0001] The present invention relates to a method for identifying and comparing plant medicinal materials, especially a method suitable for identifying and comparing specific varieties of plant medicinal materials by chloroplast DNA sequence. Background technique [0002] The origin identification of Chinese medicinal materials has always been tested by source, traits, microscopic examination and physical and chemical methods. This has considerable credibility in mineral medicine or animal medicine, but for some special parts such as tissues or organs, or has lost the original after processing The traits of medicinal materials cannot be distinguished. The appearance of the Chinese medicinal materials on the market are similar, the same name is different, or the same name is different. It is very common to cause misuse and mixed use. In addition, some noble medicinal materials are fraudulently used seriously, causing the problem of low efficacy and uncontrollable ef...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/50G01N33/68
Inventor 邱淑娇李文静李承榆颜瑞宏李专义李连滋
Owner IND TECH RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com