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Hair weeds bacterial culture and culturing product

A cell culture and cell technology, applied in the direction of plant cells, fermentation, unknown raw materials, etc., can solve the problems of ecological environment deterioration, difficult development, vegetation destruction, etc., and achieve the effect of protecting the ecological environment and saving natural resources

Inactive Publication Date: 2004-09-22
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The unreasonable harvesting of Nostocs also caused serious damage to the vegetation where the Nostocs grows, resulting in desertification of the land and the deterioration of the ecological environment
In view of this situation, the Chinese government has completely banned the harvesting, processing and sales of Nostocchia from July 2000, and the production of Nostocchia as raw material cannot be carried out. As a biologically active agent with good application prospects Substances whose development and production are difficult due to lack of sources of raw materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Separation of the Nostocella cells:

[0017] Weigh 1g of Nostocella in a triangular flask, sterilize the surface, and rinse with sterile water. Add filter-sterilized isolated enzyme solution 30mL under sterile conditions, the enzyme solution contains 0.5% pectinase, 0.8% mannitol, and 1% dextran sulfate potassium. The Erlenmeyer flask was placed on a shaker with a rotation speed of 120r / min, a stroke of 4-5cm, a temperature of 25°C, and a time of 2 hours, during which the enzyme solution was replaced every 30 minutes. After the third enzyme solution was replaced, glass beads with a diameter of 2mm were added to the Erlenmeyer flask. Two hours later, the enzymolysis solution was filtered with 800-mesh metal mesh, and the filtrate was centrifuged at 3000r / min for 15 minutes to collect the cells of Nostocchia.

[0018] (2) Cultivate the Nostocella cell species:

[0019] After washing with medium for 3 times, the Nostocella cells were inserted into the medium, the med...

Embodiment 2

[0023] Weigh 1g of Nostocchi, put it in a mortar after surface sterilization, add about 30mL of sterile saline or culture medium, grind it fully under sterile conditions, filter with 800-mesh metal mesh, centrifuge the filtrate, collect Nostoc cells, and culture Fat vegetables cell species. The cell species were preserved at 10°C, or inserted into the culture medium after expansion, the inoculum size was 20%, the pH was 9, the culture temperature was 30°C, and the light was cultivated for 15 days, and the rest were the same as in Example 1.

Embodiment 3

[0025] Lettuce cells were stored at 12°C. After the expansion of the cell species, insert it into the reactor with a 15% inoculum. The composition of the medium: 1.6% sodium bicarbonate, 0.12% sodium nitrate, 0.03% ammonium nitrate, add dipotassium hydrogen phosphate, calcium chloride, magnesium sulfate, pH8 .75, 32°C, 4500lux light intensity, light-to-dark ratio 16:8, light culture for 12 days. All the other are with embodiment 1 or embodiment 2.

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Abstract

A cell culture process for flagelliforme includes such steps as separating the cells from flagelliforme frond to obtain high-activity flagelliforme cells, culturing them in culture medium to obtain its seed cells, storing them at 8-12 deg.C under 500 lux or freeze drying for long-term storage, large-scale culturing to obtain the cultured product, and extracting flagelliforme polyol.

Description

technical field [0001] The invention relates to a cell culture technology, in particular to a cell culture technology of Nostocchia and a production technology of Nostocci polysaccharides. Background technique [0002] Nostoc flagelliforme is a terrestrial cyanobacteria rich in polysaccharides, amino acids and trace elements. It has been loved by people as a valuable food for a long time. In recent years, the medicinal value of Nostoc chinensis has been paid more and more attention. The polysaccharides contained in Nostoc sativa can enhance the body's immunity, and the hot water extract of Nostoc sativa has been proved by experiments to have anti-tumor activity. Nostoflan, a polysaccharide extracted from hair vegetables, has been proved to have antiviral activity against some enveloped viruses such as type 1 herpes simplex virus, human cytomegalovirus and influenza virus, and can kill tumor cells cultured in vitro . Abroad has developed the health care product that is main...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04C12P19/04
Inventor 贾士儒苏建宁乔长晟
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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