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Lobay tricholoma strain separation pure culture technigne and cultivation method thereof

A technology for strain isolation and separation medium, which is applied in the field of isolation and pure cultivation of Tricholoma lobai strains, can solve problems such as unsatisfactory effects, inability to form entities alone, and inability to realize large-scale cultivation and production of Tricholoma lobai, and achieve high yield High, high biological efficiency

Inactive Publication Date: 2003-05-28
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 1991, Ganeshan used straw to test the artificial cultivation of Tricholoma lobai, but the effect was not satisfactory.
At present, there is no artificial cultivation of Tricholoma lobai in China, only wild Tricholoma lobai
The wild Tricholoma lobai strain is a mycorrhizal fungus symbiotic with the Phoenix tree. Under natural conditions, it cannot form an entity alone, so it cannot be cultivated in large quantities to produce Tricholoma lobai.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Weigh 60g of potatoes, cut them into small pieces, put them in a beaker, add 100ml of water, boil for 5-10 minutes, filter with gauze, take the filtrate to a constant volume of 100ml, add 2g of agar and 2g of glucose to the filtrate, pH6.5, stir and cook Dissolve and pack in test tubes, each test tube is 10ml, the test tube mouth is plugged with cotton, 9 sticks are wrapped with kraft paper on the test tube mouth end, put into a pressure cooker for sterilization, the temperature is 121-126 °C, the pressure is 1.1-1.5 kg / cm 2 When the temperature is maintained for 30 minutes, when the natural pressure drops to 0, the inclined surface is taken out, and the culture medium is prepared after the inclined surface is solidified. Under aseptic conditions, tear the fresh Tricholoma lobai in half, cut a small piece at the junction of the cap and the stipe with a sterile inoculation knife, transfer it to the slant of the medium, and place it in a clean environment to cultivate. ...

Embodiment 2

[0067] Weigh 25g of cottonseed hulls, put them into a beaker, add 100ml of water, boil for 5-10 minutes, filter with gauze, take the filtrate to a constant volume of 100ml, add 2g of agar and 2g of glucose to the filtrate, stir and boil to dissolve, and divide into test tubes , each test tube is 10ml, the test tube mouth is plugged with cotton, 9 tubes are wrapped with kraft paper on the test tube mouth end, put into a pressure cooker for sterilization, the temperature is 121-126 ℃, the pressure is 1.1-1.5kg / cm 2 When the temperature is maintained for 30 minutes, when the natural pressure drops to 0, the inclined surface is taken out, and the culture medium is prepared after the inclined surface is solidified. Under aseptic conditions, the fresh Lobay mushroom seeds are torn in half, and at the junction of the cap and the stalk, a small piece is cut with a sterile inoculation knife, transferred to the slant of the medium, and placed in a clean environment for cultivation . Th...

Embodiment 3

[0069] Weigh 1.5g sucrose, 0.5g peptone, KH 2 PO 4 0.05g, MgSO 4 Put 0.05g, 1.8g agar, and 100ml water into a beaker, boil to dissolve, and divide into test tubes, each test tube is 10ml, the test tube mouth is plugged with cotton, 9 sticks are wrapped with kraft paper on the test tube mouth end, and put into a pressure cooker Medium sterilization, temperature 121-126 ℃, pressure 1.1-1.5kg / cm 2 , maintained for 30 minutes, and when the natural pressure reduction was 0, take out the beveled surface, and after the beveled surface was solidified, under aseptic conditions, the successfully isolated wild Tricholoma lobai strains described in Examples 1 and 2 were inoculated with a sterile inoculation knife. Cut a small piece of mycelium, move it to the slant of the medium, and place it in a clean environment for cultivation. Mycelia covered with no pollution on the inclined surface of the test tube is the mother species.

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PUM

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Abstract

A technique for separating, purifying and cultivating Lobay Trichloma as an edible fungus is characterized by that the wild lobay Tricholoma strain is naturalized as the strain able to be individiaul cultured by choosing different culture media and culture conditions. Its advantages are high biological conversion (100%) and high yield of new strain.

Description

Technical field: [0001] The invention belongs to the technical field of cultivation of edible fungi, in particular to a technique for the isolation and pure cultivation of trichomonas lobai strains and a cultivation method thereof. Background technique: [0002] Tricholoma lobyense Heim, also known as Tricholoma lobyense Heim, is a tropical fungus belonging to Basidiomycotina, Agaricales, Tricholomaceae, and Tricholoma. Rhizopus. The fungus is non-toxic, edible, delicious, thick and tender, and has high economic value. It is generally believed that Tricholoma is almost all mycorrhizal fungi, and artificial cultivation is more difficult. Japan has a history of nearly 100 years of research on the domestication and cultivation of matsutake mushrooms, and it is still in a semi-artificial cultivation state. In 1991, Ganeshan used straw to do artificial cultivation of Tricholoma lobai, but the effect was not ideal. At present, there is no artificial cultivation of Tricholoma l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 刘月廉郭荣发谭树明温美英
Owner GUANGDONG OCEAN UNIVERSITY
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