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Pinellia tuber total organic acid and extraction method, and its use in preparing drugs

A technology of organic acids and Pinellia, applied in organic chemistry methods, purification/separation/stabilization of organic compounds, chemical instruments and methods, etc., can solve the problem of unclear effective parts and active ingredients, and achieve obvious antitussive effect. Significant effect, inhibitory effect

Inactive Publication Date: 2006-11-08
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the problem that the effective parts and active ingredients of Pinellia medicinal materials that have therapeutic effects are still unclear. The patent application of the present invention is to extract the effective parts and effective Components, as well as extraction and separation methods and pharmacological effects, and their application in the preparation of anti-cough and phlegm-reducing, anti-tumor drugs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: the extraction method of pinellia total organic acid

[0031] (1) Take by weighing 5Kg of the raw medicinal material Pinellia, grind it into a coarse powder, and pass through a 14-mesh sieve;

[0032] (2) heat and reflux twice with 10 times the amount of 75% ethanol, each time for 1.5 hours, and recover the ethanol under reduced pressure until there is no ethanol smell, to obtain alcoholic extract;

[0033] (3) Add dilute hydrochloric acid to adjust the pH to 2.0, extract the solution with ethyl acetate (according to 1:3) until the extract is colorless, and concentrate under reduced pressure;

[0034] (4) With 5% anhydrous Na 2 CO 3 Solution extraction, acidify with 20% hydrochloric acid after combining lye;

[0035] (5) Extract with ethyl acetate (according to 1:3) until the extraction is colorless and there is no organic acid reaction, and the extract is decompressed to recover ethyl acetate to obtain the extract extract;

[0036] (6) After dissolvin...

Embodiment 2

[0037] Embodiment 2: the separation of total organic acid component

[0038] Mix 50g of total organic acid extract and 120g of silica gel as a sample, put it on the column by wet method, and use petroleum ether: ethyl acetate for gradient elution according to different ratios, and concentrate the eluent at the ratio of petroleum ether: ethyl acetate 20:1 After standing to crystallize to obtain palmitic acid; in the ratio of petroleum: ethyl acetate 1:1, the eluate is concentrated and then statically crystallized and recrystallized with methanol to obtain succinic acid; in the ratio of petroleum: ethyl acetate 1:5 The eluate was concentrated and crystallized on standing to obtain N-acetylglutamic acid. In addition, dissolve 1 g of pinellia total organic acid extract in ethanol solution, spread the plate with silica gel G, dry it and place it in a chromatography tank in a saturated potassium nitrate solution for 10 hours, and apply 300 ul of the organic acid solution as a strip ...

Embodiment 3

[0040] Example 3, the influence of pinellia total organic acids on the number of coughs and incubation period in mice induced by ammonia water and sulfur dioxide

[0041] 1. Experimental materials

[0042] 1.1 Sample, ① Pinellia total organic acid extract, prepared into a solution with a certain concentration before use. Preparation method: Take an appropriate amount of extract, weigh it accurately, put it in a mortar, add 0.1ml Tween-80, grind it thoroughly, then take 10ml normal saline and add it in several times, grind it while adding, until it is fully ground evenly. ② Cough must be clear.

[0043] 1.2 Reagents, ammonia water, anhydrous sodium sulfite, sulfuric acid.

[0044] 1.3 Instrument, 402 Ultrasonic Nebulizer

[0045] 1.4 Animals, ICR mice, half male and half male, 18-22g, provided by the Animal Center of Nanjing University of Traditional Chinese Medicine.

[0046] 2. Methods and Results

[0047]40 mice, half male and half female, body weight 20 ± 2g, were rand...

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PUM

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Abstract

The invention discloses a part of total organic acid extracted from the Chinese medicinal material Pinellia Pinelliae, which has the functions of relieving cough, reducing phlegm, eliminating mass and resolving stagnation, and is separated from the total organic acid to obtain succinic acid, palmitic acid and N-acetylglutamic acid. , malic acid four active ingredients. The extraction method is to crush the pinellia medicinal material into a coarse powder, extract with ethanol, extract with ethyl acetate, lye, and ethyl acetate successively, concentrate under reduced pressure to obtain the extract, dissolve the extract with ethanol, filter, and decompress Recover ethanol to obtain total organic acid extract. Then mix the extract with silica gel, put it on the column by wet method, and get succinic acid, palmitic acid, N-acetylglutamic acid after gradient elution of petroleum ether-ethyl acetate, and four kinds of malic acid and so on are obtained by preparative chromatography. Active ingredients. The experimental results show that total organic acids have significant effects of relieving cough and reducing phlegm, anti-inflammatory and antibacterial, promoting gastric motility and inhibiting tumor cell growth.

Description

1. Technical field: [0001] The invention relates to a Chinese herbal medicine extract, in particular to a Chinese herbal medicine extract, an extraction method and its application in preparing medicines for treating diseases. 2. Background technology: [0002] Pinellia is the dry tuber of Pinellia ternata (Thunb) Breit, a plant of the family Araceae. It is the most commonly used traditional Chinese medicine in clinical practice. Clinically, it is mainly used in combination with various prescriptions to treat cough with excessive phlegm, dyspnea with phlegm, dizziness and palpitations, vomiting and nausea, fullness in the chest and abdomen, plum nuclear gas, phlegm and headache, etc. The raw product is used externally to treat carbuncle and swollen sputum nucleus, and it is often used after ginger preparation Yu Jiangni antiemesis. [0003] Pinellia is mainly produced in Sichuan, Jiangsu, Anhui, Zhejiang, Henan, Hubei, Jiangxi, Shandong, Yunnan and other places. Wild Pinelli...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07B63/00A61K36/00A61P11/14A61P11/10A61P31/06A61P35/00
Inventor 吴皓李伟何凌云王媚池玉梅张科卫许凤清
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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