Pinellia tuber total organic acid and extraction method, and its use in preparing drugs
A technology of organic acids and Pinellia, applied in organic chemistry methods, purification/separation/stabilization of organic compounds, chemical instruments and methods, etc., can solve the problem of unclear effective parts and active ingredients, and achieve obvious antitussive effect. Significant effect, inhibitory effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0030] Embodiment 1: the extraction method of pinellia total organic acid
[0031] (1) Take by weighing 5Kg of the raw medicinal material Pinellia, grind it into a coarse powder, and pass through a 14-mesh sieve;
[0032] (2) heat and reflux twice with 10 times the amount of 75% ethanol, each time for 1.5 hours, and recover the ethanol under reduced pressure until there is no ethanol smell, to obtain alcoholic extract;
[0033] (3) Add dilute hydrochloric acid to adjust the pH to 2.0, extract the solution with ethyl acetate (according to 1:3) until the extract is colorless, and concentrate under reduced pressure;
[0034] (4) With 5% anhydrous Na 2 CO 3 Solution extraction, acidify with 20% hydrochloric acid after combining lye;
[0035] (5) Extract with ethyl acetate (according to 1:3) until the extraction is colorless and there is no organic acid reaction, and the extract is decompressed to recover ethyl acetate to obtain the extract extract;
[0036] (6) After dissolvin...
Embodiment 2
[0037] Embodiment 2: the separation of total organic acid component
[0038] Mix 50g of total organic acid extract and 120g of silica gel as a sample, put it on the column by wet method, and use petroleum ether: ethyl acetate for gradient elution according to different ratios, and concentrate the eluent at the ratio of petroleum ether: ethyl acetate 20:1 After standing to crystallize to obtain palmitic acid; in the ratio of petroleum: ethyl acetate 1:1, the eluate is concentrated and then statically crystallized and recrystallized with methanol to obtain succinic acid; in the ratio of petroleum: ethyl acetate 1:5 The eluate was concentrated and crystallized on standing to obtain N-acetylglutamic acid. In addition, dissolve 1 g of pinellia total organic acid extract in ethanol solution, spread the plate with silica gel G, dry it and place it in a chromatography tank in a saturated potassium nitrate solution for 10 hours, and apply 300 ul of the organic acid solution as a strip ...
Embodiment 3
[0040] Example 3, the influence of pinellia total organic acids on the number of coughs and incubation period in mice induced by ammonia water and sulfur dioxide
[0041] 1. Experimental materials
[0042] 1.1 Sample, ① Pinellia total organic acid extract, prepared into a solution with a certain concentration before use. Preparation method: Take an appropriate amount of extract, weigh it accurately, put it in a mortar, add 0.1ml Tween-80, grind it thoroughly, then take 10ml normal saline and add it in several times, grind it while adding, until it is fully ground evenly. ② Cough must be clear.
[0043] 1.2 Reagents, ammonia water, anhydrous sodium sulfite, sulfuric acid.
[0044] 1.3 Instrument, 402 Ultrasonic Nebulizer
[0045] 1.4 Animals, ICR mice, half male and half male, 18-22g, provided by the Animal Center of Nanjing University of Traditional Chinese Medicine.
[0046] 2. Methods and Results
[0047]40 mice, half male and half female, body weight 20 ± 2g, were rand...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com