Polycondensed amino-acid coordinated SOD simulatives
An amino acid and mimetic technology, applied in the field of superoxide dismutase analogs, can solve the problems of reduced capacity, poor lipid solubility, and reduced SOD-like activity, and achieves moderate molecular weight, broad application prospects, and good water solubility.
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Embodiment 1
[0024] Weigh 2.0 g of aspartic acid polycondensate with an average molecular weight of 3000 and dissolve it in 15 mL of 1 mol / L ammonia water. The solution is a dark red transparent solution, and the pH value is adjusted to 4 with 10 mol / L hydrochloric acid solution. In addition, 1.5 g of ferrous sulfate and equimolar hydroxylamine hydrochloride were weighed and dissolved in 15 mL of distilled water. Mix the two solutions in a 250mL ground-mouth Erlenmeyer flask. After mixing, the system is still a dark red transparent solution. Put the reaction system in a constant temperature water bath, gradually raise the temperature to 80°C, and take it out after magnetic stirring for 6 hours. The system is still deep red. Red transparent solution, cooled to room temperature. After overnight at 4°C, flocculent suspended matter appeared, and the precipitate after suction filtration was washed twice with distilled water, and dried naturally to obtain a light orange powder with a yield of ab...
Embodiment 2
[0026] Weigh 2.0 g of the aspartic acid polymer and dissolve it in 15 mL of 1 mol / L ammonia water, and adjust the pH value to 7 with 10 mol / L hydrochloric acid solution. Separately weigh 1.7 g of manganese sulfate and dissolve it in 15 mL of distilled water. The two solutions were mixed in a 250 mL ground-mouth Erlenmeyer flask. After mixing, the system was a deep red transparent solution. After magnetic stirring and reflux for 6 hours, the system was still a deep red transparent solution and cooled to room temperature. After overnight at 4°C, flocculent suspended matter appeared, and the filtered precipitate was washed twice with distilled water and air-dried naturally to obtain a white powder. The yield is about 60%.
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