Production method of transplanting material

Abstract

The present invention relates to a method for manufacturing a graft material used for the implantation of a biological corneal stromal ring (ICR), comprising the following steps: Step 1, preparing a biological scaffold; Step 2, separating and culturing biological corneal stromal cells; and Step 3 1. Prepare corneal stromal cell-biological scaffold complex and prefabricate corneal stromal tissue; it may further include step 4, implanting the forming cell-biological material compound obtained in step 3 into the corneal stromal lamina of the living body to obtain newborn Tissue engineered corneal stromal tissue. The corneal stroma tissue obtained by the present invention is implanted into the periphery of the living corneal stroma to increase the thickness of the cornea to treat ametropia. Compared with the ICR using other polymer materials in the prior art, it has a more reliable effect, is more stable, and is expected Sex is better. Moreover, the real biological healing can be achieved between this material and the cornea, completely avoiding complications such as corneal dissolution, aqueous humor leakage, and implant prolapse that may be caused by other alternative material transplants.

Description

technical field [0001] The present invention relates to a method for manufacturing a graft material, in particular to a method for manufacturing a graft material constructed by tissue engineering, which is applied to implantation of intrastromal corneal ring (ICR) in a living body. Background technique [0002] In recent years, there are generally many kinds of corneal refractive surgery for correcting the diopter of biological eyes adopted by the ophthalmology field, such as radial keratotomy (RK), excimer laser keratectomy (PRK), automatic lamellar keratoplasty (ALK) ), and laser in situ keratomileusis (LASIK), etc., there are many types of operations, each with its own strengths. The evaluation criteria for clinical practice of refractive surgery are usually effectiveness, predictability, stability and safety, and the selection of surgical methods must comprehensively estimate the risk-benefit ratio. RK, PRK, ALK, and LASIK are relatively mature and have good clinical ef...

AI Technical Summary

Problems solved by technology

However, the polymer materials used, such as PMMA, are mostly non-permeable, non-flexible and non-porous materials. The real biological healing between such materials and the living cornea cannot be achieved, and may eventually lead to corneal dissolution, Late complications such as aqueous humor leakage and implant prolapse

Moreover, the long-term existence of such polymer materials (such as PMMA) in the cornea of ​​organisms may cause other adverse biological reactions that need further observation and research.

[0005] Due to the insecurity of the source of ICR materials required for ICR implantation surgery, it has become a serious obstacle restricting the further widespread development of this technology.

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