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Method for discriminating medicinal material mixing and establishing chloroplast DNA data bank of medicinal material

A chloroplast and database technology, which is applied in the direction of material inspection products, biochemical equipment and methods, and microbial measurement/inspection, and can solve problems such as mixed use, fraudulent use, and misuse of medicinal materials

Inactive Publication Date: 2005-11-23
IND TECH RES INST
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  • Claims
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Problems solved by technology

[0004] At present, the market of traditional Chinese medicinal materials has been investigated by scholars and experts, and it is found that various medicinal materials are misused, mixed and used fraudulently.

Method used

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  • Method for discriminating medicinal material mixing and establishing chloroplast DNA data bank of medicinal material
  • Method for discriminating medicinal material mixing and establishing chloroplast DNA data bank of medicinal material

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Comparison scheme
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Embodiment approach

[0059] The non-coding spacer used in the method of the present invention can be any non-coding spacer in the chloroplast DNA map. When applied to Scutellaria, it is preferably a non-coding spacer located between the psbA gene and the trnH gene; There are no restrictions on the plant medicinal materials applicable to the method; the following primer pairs are preferred in the method of the present invention:

[0060] 5’-TGATCCACTTGGCTACATCCGCC-3’

[0061] 5’-GCTAACCTTGGTATGGAAGT-3’

[0062] The polymerase chain reaction was performed on the non-coding spacer between the psbA gene and the trnH gene in the chloroplast DNA fragments extracted from Scutellaria baicalensis Georgi.

[0063] In order to enable the examiner to better understand the technical content of the present invention, a preferred specific embodiment is described as follows.

[0064] The scutellaria medicinal materials used in this example were collected from various places.

[0065] (1) The medicinal materials are c...

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Abstract

The plant medicinal material identifying and comparing method includes mainly the following steps: providing the first chloroplast DNA sequence of the first plant medicinal material sample; provides the second plant medicinal material sample; extracting the total DNA from the sample; PCR proliferating the extracted plant medicinal chloroplast DNA segment containing one nonsense meson; sorting the chloroplast DNA segment to obtain the chloroplast DNA sequence of the second sample; and comparing the first chloroplast DNA sequence and the second chloroplast DNA sequence to determine whether the second sample plant medicinal material is the same as the first one.

Description

Technical field [0001] The present invention relates to a method for identifying and comparing plant medicinal materials, especially a method suitable for identifying and comparing specific varieties of plant medicinal materials by chloroplast DNA sequence. Background technique [0002] The origin identification of Chinese medicinal materials has always been tested by source, traits, microscopic examination and physical and chemical methods. This has considerable credibility in mineral medicine or animal medicine, but for some special parts such as tissues or organs, or has lost the original after processing The traits of medicinal materials cannot be distinguished. The appearance of the Chinese medicinal materials on the market are similar, the same name is different, or the same name is different. It is very common to cause misuse and mixed use. In addition, some noble medicinal materials are fraudulently used seriously, causing the problem of low efficacy and uncontrollable ef...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68G01N33/50G01N33/68
Inventor 邱淑娇李文静李承榆颜瑞宏李专义李连滋
Owner IND TECH RES INST
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