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Prepn and use of enzyme prepn for use in hemp degluing industrial process

An industrial enzyme and degumming technology, applied in the field of microbial engineering, can solve the problems of environmental pollution, difficult to stabilize the effect, low production rate, etc., and achieve the effect of improving working conditions

Inactive Publication Date: 2005-02-09
山东省纤维检验局 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Hemp degumming has been "transplanting" the ramie degumming process for more than ten years. The disadvantages are: environmental pollution and uneven "raw and cooked" degummed fibers, and the production rate is low
Obviously, this is still natural retting under artificial control conditions, the effect is difficult to stabilize, and industrial application is very difficult

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: the preparation method of hemp degumming industrial enzyme preparation

[0023] The degumming enzyme liquid is produced according to the three-stage fermentation process: the first-level seed tank is 50L, the second-level seed tank is 500L, and the fermentation tank is 10M 3 .

[0024] CCTCC No.M200038 bacterial slant strains were inoculated into the shake flask enzyme production medium. The preparation method is as follows: 50 ml of the enzyme-producing medium in a 300 ml shake flask is placed at 32° C.±1° C. and cultured on a shaker at 200 rpm for 72 hours.

[0025] Medium for cultivating potato slant: 100 parts of 20% by weight potato juice, 1.5 parts of sucrose, 0.3 part of pectin, 0.05 part of yeast extract, and 1.6 parts of agar. Both are parts by weight, the same below.

[0026] Medium for shake flask culture: (1) 3.0 parts of bran, 1.0 part of corn flour, (NH 4 ) 2 SO 4 1.0 copies, K 2 HPO 4 0.1 parts, MgSO 4 ·7H 2 O 0.1 part, yeast extr...

Embodiment 2

[0029] Embodiment 2: the preparation method of industrial enzyme preparation for hemp degumming.

[0030] As described in Example 1, the difference is:

[0031] The culture medium of the slope is the culture medium of the beef peptone agar slope: 0.3 parts of beef extract, 0.5 parts of peptone, 0.5 parts of NaCl, 2.0 parts of agar, and 100 parts of water.

[0032] Shake flask culture medium: 0.3 parts of beef extract, 0.5 parts of peptone, 0.5 parts of NaCl, 0.8 parts of sucrose, and 100 parts of water.

[0033] The degumming enzyme liquid is produced according to the first-level seed tank and fermentation tank process: the first-level seed tank is 50L, and the fermentation tank is 10M 3 .

Embodiment 3

[0034] Embodiment 3: the cannabis degumming steps are as follows:

[0035] (1) Degumming enzyme liquid as described in embodiment 1, after diluting 5 times with water, adjust pH8.5.

[0036] (2) Hemp roots are cut to 150mm, tied, dried and softened twice, and soaked in water at 90°C and pH 8 for 1 hour.

[0037] (3) The bath ratio of cannabis to the diluted enzyme solution is 1:18 (weight: volume), and the degumming is carried out in a water bath at 40° C. for 6 hours, and the pH is 8. Each cage contains 320Kg of marijuana, divided into three cages, and three batches of degumming total 960Kg.

[0038] After taking it out for washing, dehydration, fluffing, oiling, degreasing, fluffing, drying, softening, stacking for health preservation. According to the test method of chemical degumming hemp strip analysis test results: the average length is 93mm, the average fineness is 19.23dtex, and the breaking strength is 3.6g / D.

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Abstract

The present invention relates to the field of microbial engineering technology. The enzyme preparation is prepared through the process including slant culture and storing, shaking culture, seeding tank culture and fermenting tank production of high enzyme activity bacteria strain. Bacillus subtilis of No.13 strain and preservation number CCTCC No.M200038 is selected. The hemp degumming process includes the fermentation of enzyme liquid filtering residue, diluting bacteria liquid to 3-6 times, regulating the pH value of fermented enzyme liquid in degluing tank to 7.5-9.0, hemp material pre-treatment, degumming with degumming liquid for 2-10 hr. The process of the present invention has no environmental pollution, and can produce hemp top with length, fineness and breaking strength superior to those of chemical process.

Description

(1) Technical field [0001] The invention relates to a preparation method and application of an efficient industrial enzyme preparation for hemp degumming, belonging to the technical field of microbial engineering. (2) Background technology [0002] Hemp (Cannabis stative) is an annual herbaceous plant belonging to the family Cannabis genus and is one of the oldest fiber crops in the world. The main components of its bast are cellulose, hemicellulose, pectin, lignin and crude protein. Not only the gum composition is complex, but also the content is high, especially the lignin content is about 5 to 8 times that of ramie and 2 to 3 times that of flax. Hemp fibers are generally 7mm to 50mm long and 14μm to 17μm wide. Using modern equipment to weave hemp has always been a difficult problem in the world. [0003] In order to make hemp spinning possible, only its technical fibers can be used, which will remove the colloid between the fiber bundles and decompose the fiber bundles...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12S3/00C12S3/02D01C1/04
Inventor 殷立德刘自镕周明琼孙中爱任建平刘兴荣朱林孙培敏周桂芬魏守文王侠冯瑞良金宗武谢小济
Owner 山东省纤维检验局
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