Plant expression vector and application thereof in degradation of rice lignin

Abstract

The invention belongs to the technical field of bioengineering, and particularly relates to a plant expression vector and application thereof in degradation of rice lignin. According to the plant expression vector, a GRP gene, an SHP gene and a GLOX gene are connected to a pCAMBIA3301 vector, the sequence of the GRP gene is as shown in SEQ ID NO.4, the sequence of the SHP gene is as shown in SEQ ID NO.3, and the GLOX gene is as shown in SEQ ID NO.7. The invention further discloses a preparation method of the plant expression vector. When the plant expression vector is used for transforming rice, the lignin content of the obtained transgenic rice in the full ripening stage is reduced, the lignin content change in the flowering stage is not large, and the problems that lignin is lost in the crop growth process, and popularization and application of a biological treatment method in production are limited are solved.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a plant expression vector and its application in degrading rice lignin. Background technique [0002] Lignocellulosic resources are abundant renewable energy materials in nature. As the main carrier of biomass energy, their research and application have been widely concerned at home and abroad. Lignin is one of the three main components of the plant cell wall. It is surrounded or bound in the cellulose framework, which can enhance the mechanical strength of the plant body, and at the same time is beneficial to the water transport of the transport tissue and resist the adverse invasion of the external environment. The complexity of its structure and its stable form in vivo make it difficult to be degraded, industrially separated or transformed, and it has become the primary barrier for the utilization of lignocellulose resources. [0003] The key to improving t...

AI Technical Summary

Problems solved by technology

Although microorganisms such as white rot fungi have the ability to degrade lignin, due to the long culture period of the strains, the yield and activity of the enzyme are low, and the process of degrading lignin by white rot fungi will cause the loss of lignin during the growth of crops. Greatly limit the popularization and application of environment-friendly biological pretreatment method in production

[0005] In summary, the problem in the prior art is that there is a lack of a method that does not cause lignin loss during crop growth and can effectively degrade straw lignin after crop harvesting

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